Sandbox Reserved 822: Difference between revisions
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=== Interactions with Phosphatidylinositol Phosphates === | === Interactions with Phosphatidylinositol Phosphates === | ||
[[Image:PDK1 PH Domain Interacting With diC4-PtdIns(3,4,5)P3.jpg| | [[Image:PDK1 PH Domain Interacting With diC4-PtdIns(3,4,5)P3.jpg|right|320px|thumb|Fig.3 Stereo representation of the PDK1 PH domain interacting with diC4-PtdIns(3,4,5)P<sub>3</sub> (marine). Under the semitransparent surface, the conserved Arg residues (green) contacting the D1- and D3- phosphates are drawn as a stick representation.<ref name="Structural" />]] | ||
The interations of the PDK1 PH domain with phosphatidylinositol phosphates were investigated by co-crystallising the PH domain with a PtdIns(3,4,5)P<sub>3</sub> analogue which contains two C4 acyl chains (diC4-PtdIns(3,4,5)P<sub>3</sub>). It was found that <scene name='56/568020/Arg472/1'>Arg472</scene> coordinates the free oxygen atoms on the D1-phosphate whereas the oxygen atom involved in the ester bond to the glycerol does not make any significant contact with the protein. The glycerol backbone itself projects away from the surface of the protein and does not display any other interactions (see Fig.3). <ref name="Structural" /> | The interations of the PDK1 PH domain with phosphatidylinositol phosphates were investigated by co-crystallising the PH domain with a PtdIns(3,4,5)P<sub>3</sub> analogue which contains two C4 acyl chains (diC4-PtdIns(3,4,5)P<sub>3</sub>). It was found that <scene name='56/568020/Arg472/1'>Arg472</scene> coordinates the free oxygen atoms on the D1-phosphate whereas the oxygen atom involved in the ester bond to the glycerol does not make any significant contact with the protein. The glycerol backbone itself projects away from the surface of the protein and does not display any other interactions (see Fig.3). <ref name="Structural" /> | ||