User:Christina Manner/Sandbox 810: Difference between revisions

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=='''Biological and Biotechnological Relevance'''==

TraR is the activator for the conjugal transfer of Ti-plamids <ref name = Hiei> Hiei et al. (1994):'' Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium tumefaciens and sequence analysis of the boundaries of the T-DNA''. In: ''The Plant Journal'' 6(2); 271-282; PMID:7920717 </ref>. In nature, ''Agrobacterium tumefaciens'' uses the transfer of the Ti-plasmid to manipulate dicotyledonous plants into producing metabolites as nutrients for the bacteria <ref name = Piper/>. TraR activates the ''tra'' genes if AAI is present and also activates the transcription of ''traR'' and ''traI''. The ''tra'' genes are responsible for the conjugal transfer.

TraR is the activator for the conjugal transfer of Ti-plamids <ref name = Hiei> PMID: 7920717 </ref>. In nature, ''Agrobacterium tumefaciens'' uses the transfer of the Ti-plasmid to manipulate dicotyledonous plants into producing metabolites as nutrients for the bacteria <ref name = Piper/>. TraR activates the ''tra'' genes if AAI is present and also activates the transcription of ''traR'' and ''traI''. The ''tra'' genes are responsible for the conjugal transfer.

In biotechnology, the whole transfer system of the Ti-plasmid can be used to transfer DNA into dicotelydonous plant cells and to integrate that DNA into the plant genome. Naturally, ''Agrobacterium tumefaciens'' only infects dicotelydonous plants and the systems to perform this are well established. But there were also some experiments that proved a transformation using the ti-plasmid in monocotyledons <ref name = Hiei/>.</div>

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 =='''Biological and Biotechnological Relevance'''==
-TraR is the activator for the conjugal transfer of Ti-plamids <ref name = Hiei> Hiei et al. (1994):'' Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium tumefaciens and sequence analysis of the boundaries of the T-DNA''. In: ''The Plant Journal'' 6(2); 271-282; PMID:7920717 </ref>. In nature, ''Agrobacterium tumefaciens'' uses the transfer of the Ti-plasmid to manipulate dicotyledonous plants into producing metabolites as nutrients for the bacteria <ref name = Piper/>. TraR activates the ''tra'' genes if AAI is present and also activates the transcription of ''traR'' and ''traI''. The ''tra'' genes are responsible for the conjugal transfer.
+TraR is the activator for the conjugal transfer of Ti-plamids <ref name = Hiei> PMID: 7920717 </ref>. In nature, ''Agrobacterium tumefaciens'' uses the transfer of the Ti-plasmid to manipulate dicotyledonous plants into producing metabolites as nutrients for the bacteria <ref name = Piper/>. TraR activates the ''tra'' genes if AAI is present and also activates the transcription of ''traR'' and ''traI''. The ''tra'' genes are responsible for the conjugal transfer.
 In biotechnology, the whole transfer system of the Ti-plasmid can be used to transfer DNA into dicotelydonous plant cells and to integrate that DNA into the plant genome. Naturally, ''Agrobacterium tumefaciens'' only infects dicotelydonous plants and the systems to perform this are well established. But there were also some experiments that proved a transformation using the ti-plasmid in monocotyledons <ref name = Hiei/>.</div>