Sandbox Reserved 818: Difference between revisions
Lea Clusan (talk | contribs) |
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Xu, Y., & Barbieri, J. T. (1995). Pertussis toxin-mediated ADP-ribosylation of target proteins in Chinese hamster ovary cells involves a vesicle trafficking mechanism. Infection and immunity, 63(3), 825-832. | Xu, Y., & Barbieri, J. T. (1995). Pertussis toxin-mediated ADP-ribosylation of target proteins in Chinese hamster ovary cells involves a vesicle trafficking mechanism. Infection and immunity, 63(3), 825-832. | ||
</ref>. | </ref>. | ||
Electron microscopy studies have shown that PTX enters the cells via ''coated pits'' | Electron microscopy studies have shown that PTX enters the cells via ''coated pits'' | ||
<ref name="Schmidt MA (1997)"> | <ref name="Schmidt MA (1997)"> | ||
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</ref>. | </ref>. | ||
These observations imply that the holotoxin traffics via the endosomal pathway and Golgi apparatus to the endoplasmic reticulum, where it meet ATP and disulphide isomerase, leading to the release of the S1 subunit. S1 then translocate directly through the endoplasmic reticulum membrane into the cytosol | These observations imply that the holotoxin traffics via the endosomal pathway and Golgi apparatus to the endoplasmic reticulum, where it meet ATP and disulphide isomerase, leading to the release of the S1 subunit. S1 then translocate directly through the endoplasmic reticulum membrane into the cytosol. | ||
==Mechanism of pertussis toxin== | ==Mechanism of pertussis toxin== | ||