2bj7: Difference between revisions
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Revision as of 17:34, 30 October 2007
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NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH-AFFINITY SITES
OverviewOverview
The Pyrococcus horikoshii OT3 genome contains a gene (PH0601 or nikR), encoding a protein (PhNikR) that shares 33.8% amino acid sequence identity, with Escherichia coli nickel responsive repressor NikR (EcNikR), including, many residues that are functionally important in the E.coli ortholog. We, succeeded in crystallization and structural characterization of PhNikR in, the apo form and two nickel bound forms that exhibit different, conformations, open and closed. Moreover, we have identified a putative, "low-affinity" nickel-binding pocket in the closed form. This binding site, has unusual nickel coordination and exhibits high sensitivity to phosphate, in the crystal structure. Analysis of the PhNikR structures and, structure-based mutational studies with EcNikR reveals a plausible, ... [(full description)]
About this StructureAbout this Structure
2BJ7 is a [Single protein] structure of sequence from [Pyrococcus horikoshii] with NI, CL, EDO and PG4 as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structure of Pyrococcus horikoshii NikR: nickel sensing and implications for the regulation of DNA recognition., Chivers PT, Tahirov TH, J Mol Biol. 2005 May 6;348(3):597-607. PMID:15826657
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