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OCA, Sarah Welly

Revision as of 20:52, 7 January 2014 view source Sarah Welly (talk \| contribs) 119 edits No edit summary ← Older edit		Revision as of 20:53, 7 January 2014 view source Sarah Welly (talk \| contribs) 119 edits No edit summary Newer edit →
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	The <scene name='56/568011/Pous/1'>POU specific domain </scene> is located on the N-terminal site of the Oct-4 sequence and precisely from the 131th amino acid to the 205th. The POU-specific domain is able to bind autonomously the DNA with the binding consensus : [gAATAT(G/T)CA]. Thanks to a [http://en.wikipedia.org/wiki/Helix-turn-helix.com Helic-turn-helix]specific motif. As its name suggests, this pattern is composed of two α helix [http://en.wikipedia.org/wiki/Alpha_helixlinked] by an amino acids sequence which corresponds to the turn. The second helix is the most involved in the DNA binding. Indeed, this helix is able to carry out hydrogen bonds and Van der Waals interactions with bases in the major groove of DNA. The first helix allows to stabilize the complex DNA-protein. In the POU specific domain of the transcription factor Oct-4, there are 2 HTH motifs linked by another alpha helix. The HTH motif is responsible of the DNA binding thanks to the first amino acid located in every helix in HTH. Indeed, this amino acid is a		The <scene name='56/568011/Pous/1'>POU specific domain </scene> is located on the N-terminal site of the Oct-4 sequence and precisely from the 131th amino acid to the 205th. The POU-specific domain is able to bind autonomously the DNA with the binding consensus : [gAATAT(G/T)CA]. Thanks to a [http://en.wikipedia.org/wiki/Helix-turn-helix.com Helic-turn-helix]specific motif. As its name suggests, this pattern is composed of two α helix [http://en.wikipedia.org/wiki/Alpha_helixlinked] by an amino acids sequence which corresponds to the turn. The second helix is the most involved in the DNA binding. Indeed, this helix is able to carry out hydrogen bonds and Van der Waals interactions with bases in the major groove of DNA. The first helix allows to stabilize the complex DNA-protein. In the POU specific domain of the transcription factor Oct-4, there are 2 HTH motifs linked by another alpha helix. The HTH motif is responsible of the DNA binding thanks to the first amino acid located in every helix in HTH. Indeed, this amino acid is a
	<scene name='56/568011/Glutamic_acide/1'>glutamic acid</scene> which is able to interact with the backbone phosphate and the adenine base in the DNA binding site. Thanks to bioinformatics tools, alignments were carried out. Very good alignment between the Oct-4 POU domain and the ~~Lambda phage~~ [http://en.wikipedia.org/wiki/Lambda_phage] POU domain was thus discovered.		<scene name='56/568011/Glutamic_acide/1'>glutamic acid</scene> which is able to interact with the backbone phosphate and the adenine base in the DNA binding site. Thanks to bioinformatics tools, alignments were carried out. Very good alignment between the Oct-4 POU domain and the [http://en.wikipedia.org/wiki/Lambda_phage Lambda phage] POU domain was thus discovered.