Sandbox Reserved 818: Difference between revisions
Lea Clusan (talk | contribs) |
Lea Clusan (talk | contribs) |
||
| Line 36: | Line 36: | ||
labeled in blue : catalytic residues (His35, Glu129) <br /> | labeled in blue : catalytic residues (His35, Glu129) <br /> | ||
The ADP-ribosylation of trimeric G proteins occurs on a '''cystein residue''' in the ''C-terminal part of the α-subunit''. For that, the donor substrate used by PTX is '''NAD<sup>+</sup>''' and the acceptor substrate binds to the toxin through '''residues 180-219''' in the ''C-terminal region of S1''. These residues show indeed a high affinity for the G protein and are involved in the catalysis of the ADP-ribosylation. | The ADP-ribosylation of trimeric G proteins occurs on a '''cystein residue''' in the ''C-terminal part of the α-subunit''. <br /> | ||
For that, the donor substrate used by PTX is '''NAD<sup>+</sup>''', which binds the toxin through '''Trp26''', '''Arg9''' and '''Cys41''' located in the ''active site of S1''. <br /> | |||
Concerning the acceptor substrate, it binds to the toxin through '''residues 180-219''' in the ''C-terminal region of S1''. These residues show indeed a high affinity for the G protein and are involved in the catalysis of the ADP-ribosylation. | |||
==Toxic effects of pertussis toxin== | ==Toxic effects of pertussis toxin== | ||