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OCA, Sarah Welly

Revision as of 12:36, 5 January 2014 view source Sarah Welly (talk \| contribs) 119 edits No edit summary ← Older edit		Revision as of 12:44, 5 January 2014 view source Sarah Welly (talk \| contribs) 119 edits No edit summary Newer edit →
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	==Pou-homeodomain==		==Pou-homeodomain==

	The homeodomain is a very conserved 60 amino acid helix-turn helix DNA-binding domain. Its DNA sequence is called the “homeobox”, and the genes are known as “Hox gene”. The DNA recognition helix (alpha 3) binds the DNA major groove while the amino-terminal tail binds the DNA minor groove. We can draw a parallel with oct1 and oct2 about the Homeodomain and admit that the ninth residue of the DNA-recognition helix is a cysteine that might have been conserved at this position to confer DNA binding specificity , and promote more relaxed DNA sequence recognition by POU domains. The recognition helix and the inter-helix loops are rich in arginine and lysine residues, which form hydrogen bonds to the DNA backbone.		The homeodomain (POUhd) is a very conserved 60 amino acid helix-turn helix DNA-binding domain. We can find the homeodomain form the 223th to the 282th amino acid in the Oct-4 sequence.Its DNA sequence is called the “homeobox”, and the genes are known as “Hox gene”. The DNA recognition helix (alpha 3) binds the DNA major groove while the amino-terminal tail binds the DNA minor groove. We can draw a parallel with oct1 and oct2 about the Homeodomain and admit that the ninth residue of the DNA-recognition helix is a cysteine that might have been conserved at this position to confer DNA binding specificity , and promote more relaxed DNA sequence recognition by POU domains. The recognition helix and the inter-helix loops are rich in arginine and lysine residues, which form hydrogen bonds to the DNA backbone.