Sandbox Reserved 827

This Sandbox is Reserved from 06/12/2018, through 30/06/2019 for use in the course "Structural Biology" taught by Bruno Kieffer at the University of Strasbourg, ESBS. This reservation includes Sandbox Reserved 1480 through Sandbox Reserved 1543.

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TBK1 stands for Tank Binding Kinase 1. This enzyme is a cytoplasmic serine-threonine kinase, coded by EC=2.7.11.1. It catalyzes the transfer of a phosphate group from an ATP onto a protein, in order to form a phosphoprotein and an ADP. Its primary sequence is 729 residues long. This homodimere is involved in several signalization pathways such as the inhibition of apoptosis, inflammatory response. Its substrates are NF-κ-B, various IRFs (interferon regulatory factors), DDX3X. It is involved in several complexes depending on the cell type and the stimuli.

spinqualitylabelsall models TBK1 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

ProtomerProtomer

Kinase domain :

UBL Domain :

Leucine zipper :

Coiled coil :

DimerDimer

Possible residue modificationsPossible residue modifications

Several residues of TBK1 can be modified, by phosphorylation or polyubiquitination. belongs to the kinase domain and can be phosphorylated by TBK1 itself, or by another serine kinase. This phosphorylation modifies the conformation of the (residues L164-G199), making the binding of the substrate possible. When S172 is not phosphorylated, the can dock itself between two αhelix of the kinase domain of another promoter. Furthermore, an containing the residues between D167 and L173 occupies the active site of this other protomer. Therefore, the active domain is not available for the binding of the substrate. But when S172 is phosphorylated, it can bind itself on the kinase domain of its own protomer, as the HPD domain which docks in the kinase domain in an intramolecular way, coming closer to the domain. This liberates the active site of the other protomer, which can now bind a substrate. The autophosphorylation between two subunit of a dimer is not really probable, since when the protein is dimeric, the two kinase domains are located at the opposite of one another. Therefore, the phosphorylation of Ser172 is done either by the concerned protomer, either by the kinase domain of another TBK1 dimer when TBK1 are involved in scaffolding complexes. S172 can also be phosphorylated by kinases such as IKBKB or IKKB, and dephosphorylated by phosphatases such as PPM1B.

But TBK1 can undergo another type of post-translationnal modifications : polyubiquitination. Polyubiquitination in a Lys63 manner on helps the activation of the kinase. The same type of modification on is responsible for dimerization. Type Lys48 polyubiquitination on Lys670 is done by DTX4 and is responsible for the degradation of the protein.

DiseasesDiseases

Interaction with viral proteins :

TBK1 is the target of some viral proteins such as VP35 from Ebola virus, or the P protein from Borna virus. Those proteins inhibit TBK1. Normally, TBK1 is responsible for virus-induced phosphorylations that lead to the cellular antiviral state. Therefore, the inhibition of the kinase prevents the establishment of such a cellular state. Since TBK1 is involved into the virus entrance following reaction, targeting TBK1 is a great solution for the viruses to prevent the reactions from the cell.

Oncology :

In case of some cancers, the permanent activation of TBK1 may be responsible of the proliferation of the cancerous cells. Since the substrates of TBK1 are involved in the proliferation pathway when phosphorylated, this permanent activation is a reason of the non apoptosis state of cells, and therefore of their proliferation.

ReferencesReferences

Q05127