2uxb: Difference between revisions

Revision as of 19:51, 21 February 2008

CRYSTAL STRUCTURE OF AN EXTENDED TRNA ANTICODON STEM LOOP IN COMPLEX WITH ITS COGNATE MRNA GGGU IN THE CONTEXT OF THE THERMUS THERMOPHILUS 30S SUBUNIT.

OverviewOverview

During translation, some +1 frameshift mRNA sites are decoded by frameshift suppressor tRNAs that contain an extra base in their anticodon loops. Similarly engineered tRNAs have been used to insert nonnatural amino acids into proteins. Here, we report crystal structures of two anticodon stem-loops (ASLs) from tRNAs known to facilitate +1 frameshifting bound to the 30S ribosomal subunit with their cognate mRNAs. ASL(CCCG) and ASL(ACCC) (5'-3' nomenclature) form unpredicted anticodon-codon interactions where the anticodon base 34 at the wobble position contacts either the fourth codon base or the third and fourth codon bases. In addition, we report the structure of ASL(ACGA) bound to the 30S ribosomal subunit with its cognate mRNA. The tRNA containing this ASL was previously shown to be unable to facilitate +1 frameshifting in competition with normal tRNAs (Hohsaka et al. 2001), and interestingly, it displays a normal anticodon-codon interaction. These structures show that the expanded anticodon loop of +1 frameshift promoting tRNAs are flexible enough to adopt conformations that allow three bases of the anticodon to span four bases of the mRNA. Therefore it appears that normal triplet pairing is not an absolute constraint of the decoding center.

About this StructureAbout this Structure

2UXB is a Protein complex structure of sequences from Thermus thermophilus with , , and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit., Dunham CM, Selmer M, Phelps SS, Kelley AC, Suzuki T, Joseph S, Ramakrishnan V, RNA. 2007 Jun;13(6):817-23. Epub 2007 Apr 6. PMID:17416634

Page seeded by OCA on Thu Feb 21 18:51:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 4: / Line 4: @@
 ==Overview==
-During translation, some +1 frameshift mRNA sites are decoded by, frameshift suppressor tRNAs that contain an extra base in their anticodon, loops. Similarly engineered tRNAs have been used to insert nonnatural, amino acids into proteins. Here, we report crystal structures of two, anticodon stem-loops (ASLs) from tRNAs known to facilitate +1, frameshifting bound to the 30S ribosomal subunit with their cognate mRNAs., ASL(CCCG) and ASL(ACCC) (5'-3' nomenclature) form unpredicted, anticodon-codon interactions where the anticodon base 34 at the wobble, position contacts either the fourth codon base or the third and fourth, codon bases. In addition, we report the structure of ASL(ACGA) bound to, the 30S ribosomal subunit with its cognate mRNA. The tRNA containing this, ASL was previously shown to be unable to facilitate +1 frameshifting in, competition with normal tRNAs (Hohsaka et al. 2001), and interestingly, it, displays a normal anticodon-codon interaction. These structures show that, the expanded anticodon loop of +1 frameshift promoting tRNAs are flexible, enough to adopt conformations that allow three bases of the anticodon to, span four bases of the mRNA. Therefore it appears that normal triplet, pairing is not an absolute constraint of the decoding center.
+During translation, some +1 frameshift mRNA sites are decoded by frameshift suppressor tRNAs that contain an extra base in their anticodon loops. Similarly engineered tRNAs have been used to insert nonnatural amino acids into proteins. Here, we report crystal structures of two anticodon stem-loops (ASLs) from tRNAs known to facilitate +1 frameshifting bound to the 30S ribosomal subunit with their cognate mRNAs. ASL(CCCG) and ASL(ACCC) (5'-3' nomenclature) form unpredicted anticodon-codon interactions where the anticodon base 34 at the wobble position contacts either the fourth codon base or the third and fourth codon bases. In addition, we report the structure of ASL(ACGA) bound to the 30S ribosomal subunit with its cognate mRNA. The tRNA containing this ASL was previously shown to be unable to facilitate +1 frameshifting in competition with normal tRNAs (Hohsaka et al. 2001), and interestingly, it displays a normal anticodon-codon interaction. These structures show that the expanded anticodon loop of +1 frameshift promoting tRNAs are flexible enough to adopt conformations that allow three bases of the anticodon to span four bases of the mRNA. Therefore it appears that normal triplet pairing is not an absolute constraint of the decoding center.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Thermus thermophilus]]
-[[Category: Dunham, C.M.]]
+[[Category: Dunham, C M.]]
 [[Category: Joseph, S.]]
-[[Category: Kelley, A.C.]]
+[[Category: Kelley, A C.]]
-[[Category: Phelps, S.S.]]
+[[Category: Phelps, S S.]]
 [[Category: Ramakrishnan, V.]]
 [[Category: Selmer, M.]]
@@ Line 49: / Line 49: @@
 [[Category: zinc-finger]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:22:03 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:51:22 2008''