2sli: Difference between revisions
New page: left|200px<br /><applet load="2sli" size="450" color="white" frame="true" align="right" spinBox="true" caption="2sli, resolution 1.8Å" /> '''LEECH INTRAMOLECULAR ... |
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[[Image:2sli.gif|left|200px]]<br /><applet load="2sli" size=" | [[Image:2sli.gif|left|200px]]<br /><applet load="2sli" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2sli, resolution 1.8Å" /> | caption="2sli, resolution 1.8Å" /> | ||
'''LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2,7-ANHYDRO-NEU5AC, THE REACTION PRODUCT'''<br /> | '''LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2,7-ANHYDRO-NEU5AC, THE REACTION PRODUCT'''<br /> | ||
==Overview== | ==Overview== | ||
Intramolecular trans-sialidase from leech (Macrobdella decora) is the | Intramolecular trans-sialidase from leech (Macrobdella decora) is the first member of the sialidase superfamily found to exhibit strict specificity towards the cleavage of terminal Neu5Acalpha2-->3Gal linkage in sialoglycoconjugates. Its release of 2,7-anhydro-Neu5Ac instead of Neu5Ac indicates that it catalyzes an intramolecular trans-sialosyl reaction. Crystal structures of its complexes with an inactive substrate analogue 2-propenyl-Neu5Ac, and with the product 2,7-anhydro-Neu5Ac, have been determined to 1.8 A resolution. The boat conformation of the pyranose observed in the complexes supports the proposed enzymatic mechanism that O7 of an axial 6-glycerol group attacks the positively charged C2 of the intermediate. A generalized mechanism is proposed for the sialidase superfamily. | ||
==About this Structure== | ==About this Structure== | ||
2SLI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora] with SKD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] Full crystallographic information is available from [http:// | 2SLI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora] with <scene name='pdbligand=SKD:'>SKD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SLI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Macrobdella decora]] | [[Category: Macrobdella decora]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Li, S | [[Category: Li, S C.]] | ||
[[Category: Li, Y | [[Category: Li, Y T.]] | ||
[[Category: Luo, M.]] | [[Category: Luo, M.]] | ||
[[Category: Luo, Y.]] | [[Category: Luo, Y.]] | ||
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[[Category: neuraminidase]] | [[Category: neuraminidase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:17 2008'' | ||
Revision as of 19:49, 21 February 2008
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LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2,7-ANHYDRO-NEU5AC, THE REACTION PRODUCT
OverviewOverview
Intramolecular trans-sialidase from leech (Macrobdella decora) is the first member of the sialidase superfamily found to exhibit strict specificity towards the cleavage of terminal Neu5Acalpha2-->3Gal linkage in sialoglycoconjugates. Its release of 2,7-anhydro-Neu5Ac instead of Neu5Ac indicates that it catalyzes an intramolecular trans-sialosyl reaction. Crystal structures of its complexes with an inactive substrate analogue 2-propenyl-Neu5Ac, and with the product 2,7-anhydro-Neu5Ac, have been determined to 1.8 A resolution. The boat conformation of the pyranose observed in the complexes supports the proposed enzymatic mechanism that O7 of an axial 6-glycerol group attacks the positively charged C2 of the intermediate. A generalized mechanism is proposed for the sialidase superfamily.
About this StructureAbout this Structure
2SLI is a Single protein structure of sequence from Macrobdella decora with as ligand. Active as Exo-alpha-sialidase, with EC number 3.2.1.18 Full crystallographic information is available from OCA.
ReferenceReference
The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism., Luo Y, Li SC, Li YT, Luo M, J Mol Biol. 1999 Jan 8;285(1):323-32. PMID:9878409
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