4mih: Difference between revisions
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{{Large structure}} | |||
{{STRUCTURE_4mih| PDB=4mih | SCENE= }} | |||
===Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant=== | |||
{{ABSTRACT_PUBMED_24282677}} | |||
The | ==Function== | ||
[[http://www.uniprot.org/uniprot/P2OX_PHACH P2OX_PHACH]] Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). Plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H(2)O(2) for the ligninolytic peroxidases, lignin peroxidase and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose, an intermediate of a secondary metabolic pathway leading to the antibiotic cortalcerone. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.<ref>PMID:9210340</ref> <ref>PMID:8661938</ref> | |||
==About this Structure== | |||
[[4mih]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIH OCA]. | |||
==Reference== | |||
<ref group="xtra">PMID:024282677</ref><references group="xtra"/><references/> | |||
[[Category: Pyranose oxidase]] | |||
[[Category: Divne, C.]] | |||
[[Category: Fusco, L.]] | |||
[[Category: Haltrich, D.]] | |||
[[Category: Hassan, N.]] | |||
[[Category: Peterbauer, C.]] | |||
[[Category: Pisanelli, I.]] | |||
[[Category: Spadiut, O.]] | |||
[[Category: Tan, T C.]] | |||
[[Category: Flavinylation]] | |||
[[Category: Gmc oxidoreductase]] | |||
[[Category: Homotetramer]] | |||
[[Category: Hyphae]] | |||
[[Category: Oxidoreductase]] | |||
[[Category: Phbh fold]] | |||
[[Category: Pyranose 2-oxidaser oxidoreductase]] | |||
[[Category: Rossmann fold]] | |||
Revision as of 16:34, 11 December 2013
Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutantPyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant
Template:ABSTRACT PUBMED 24282677
FunctionFunction
[P2OX_PHACH] Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). Plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H(2)O(2) for the ligninolytic peroxidases, lignin peroxidase and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose, an intermediate of a secondary metabolic pathway leading to the antibiotic cortalcerone. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.[1] [2]
About this StructureAbout this Structure
4mih is a 8 chain structure. Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Hassan N, Tan TC, Spadiut O, Pisanelli I, Fusco L, Haltrich D, Peterbauer CK, Divne C. Crystal structures of pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly. FEBS Open Bio. 2013 Nov 5;3:496-504. PMID:24282677 doi:http://dx.doi.org/10.1016/j.fob.2013.10.010
- ↑ Artolozaga MJ, Kubatova E, Volc J, Kalisz HM. Pyranose 2-oxidase from Phanerochaete chrysosporium--further biochemical characterisation. Appl Microbiol Biotechnol. 1997 May;47(5):508-14. PMID:9210340
- ↑ Volc J, Kubatova E, Daniel G, Prikrylova V. Only C-2 specific glucose oxidase activity is expressed in ligninolytic cultures of the white rot fungus Phanerochaete chrysosporium. Arch Microbiol. 1996 Jun;165(6):421-4. PMID:8661938