2bf4: Difference between revisions
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[[Category: nadph-cytochrome p450 reductase]] | [[Category: nadph-cytochrome p450 reductase]] | ||
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Revision as of 17:30, 30 October 2007
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A SECOND FMN-BINDING SITE IN YEAST NADPH-CYTOCHROME P450 REDUCTASE SUGGESTS A NOVEL MECHANISM OF ELECTRON TRANSFER BY DIFLAVIN REDUCTASES.
OverviewOverview
NADPH-cytochrome P450 reductase transfers two reducing equivalents derived, from a hydride ion of NADPH via FAD and FMN to the large family of, microsomal cytochrome P450 monooxygenases in one-electron transfer steps., The mechanism of electron transfer by diflavin reductases remains elusive, and controversial. Here, we determined the crystal structure of truncated, yeast NADPH-cytochrome P450 reductase, which is functionally active toward, its physiological substrate cytochrome P450, and discovered a second FMN, binding site at the interface of the connecting and FMN binding domains., The two FMN binding sites have different accessibilities to the bulk, solvent and different amino acid environments, suggesting stabilization of, different electronic structures of the reduced flavin. Since ... [(full description)]
About this StructureAbout this Structure
2BF4 is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with SO4, FAD, FMN and NAP as [ligands]. Active as [NADPH--hemoprotein reductase], with EC number [1.6.2.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
A second FMN binding site in yeast NADPH-cytochrome P450 reductase suggests a mechanism of electron transfer by diflavin reductases., Lamb DC, Kim Y, Yermalitskaya LV, Yermalitsky VN, Lepesheva GI, Kelly SL, Waterman MR, Podust LM, Structure. 2006 Jan;14(1):51-61. PMID:16407065
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