2qh3: Difference between revisions

Revision as of 19:39, 21 February 2008

Solution structure of the U64 H/ACA snoRNA 3' terminal hairpin loop

OverviewOverview

The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.

About this StructureAbout this Structure

2QH3 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Structural and functional characterization of human telomerase RNA processing and cajal body localization signals., Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J, Mol Cell. 2007 Sep 21;27(6):869-81. PMID:17889661

Page seeded by OCA on Thu Feb 21 18:39:23 2008

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OCA

@@ Line 4: / Line 4: @@
 ==Overview==
-The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains, required for 3' end processing, localization, and accumulation. The, terminal loop of the CR7 domain contains the CAB box (ugAG) required for, targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence, required for accumulation and processing. To dissect out the contributions, of the CR7 stem loop to hTR processing and localization, we solved the, solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA, snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These, structures, together with analysis of localization, processing, and, accumulation of hTRs containing nucleotide substitutions in the CR7, domain, identified the sequence and structural requirements of the hTR, processing and CB localization signals and showed that these signals are, functionally independent. Further, 3' end processing was found to be a, prerequisite for translocation of hTR to CBs.
+The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Structural and functional characterization of human telomerase RNA processing and cajal body localization signals., Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J, Mol Cell. 2007 Sep 21;27(6):869-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17889661 17889661]
 [[Category: Protein complex]]
-[[Category: Breece, K.E.]]
+[[Category: Breece, K E.]]
 [[Category: Chim, N.]]
 [[Category: Feigon, J.]]
-[[Category: Theimer, C.A.]]
+[[Category: Theimer, C A.]]
 [[Category: g-u pair]]
 [[Category: loop]]
@@ Line 21: / Line 21: @@
 [[Category: syn g]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:34:43 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:39:23 2008''