4km9: Difference between revisions
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{{STRUCTURE_4km9| PDB=4km9 | SCENE= }} | |||
===Crystal structure of human Suppressor of Fused=== | |||
==Disease== | |||
[[http://www.uniprot.org/uniprot/SUFU_HUMAN SUFU_HUMAN]] Defects in SUFU are a cause of medulloblastoma (MDB) [MIM:[http://omim.org/entry/155255 155255]]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Defects in SUFU play a role in predisposition to desmoplastic MDB. These tumors make up about 20 to 30% of medulloblastomas, have a more nodular architecture than 'classical' medulloblastoma, and may have a better prognosis. | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/SUFU_HUMAN SUFU_HUMAN]] Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A).<ref>PMID:10564661</ref> <ref>PMID:10559945</ref> <ref>PMID:12068298</ref> <ref>PMID:10806483</ref> <ref>PMID:22365972</ref> | |||
==About this Structure== | |||
[[4km9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KM9 OCA]. | |||
==Reference== | |||
<references group="xtra"/><references/> | |||
[[Category: Qi, X.]] | |||
[[Category: Wu, G.]] | |||
[[Category: Zhang, Y.]] | |||
[[Category: Zhang, Z.]] | |||
[[Category: Protein binding]] | |||
[[Category: Suppressor of fused]] | |||
Revision as of 16:44, 20 November 2013
Crystal structure of human Suppressor of FusedCrystal structure of human Suppressor of Fused
DiseaseDisease
[SUFU_HUMAN] Defects in SUFU are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Defects in SUFU play a role in predisposition to desmoplastic MDB. These tumors make up about 20 to 30% of medulloblastomas, have a more nodular architecture than 'classical' medulloblastoma, and may have a better prognosis.
FunctionFunction
[SUFU_HUMAN] Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A).[1] [2] [3] [4] [5]
About this StructureAbout this Structure
4km9 is a 1 chain structure. Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Stone DM, Murone M, Luoh S, Ye W, Armanini MP, Gurney A, Phillips H, Brush J, Goddard A, de Sauvage FJ, Rosenthal A. Characterization of the human suppressor of fused, a negative regulator of the zinc-finger transcription factor Gli. J Cell Sci. 1999 Dec;112 ( Pt 23):4437-48. PMID:10564661
- ↑ Kogerman P, Grimm T, Kogerman L, Krause D, Unden AB, Sandstedt B, Toftgard R, Zaphiropoulos PG. Mammalian suppressor-of-fused modulates nuclear-cytoplasmic shuttling of Gli-1. Nat Cell Biol. 1999 Sep;1(5):312-9. PMID:10559945 doi:10.1038/13031
- ↑ Taylor MD, Liu L, Raffel C, Hui CC, Mainprize TG, Zhang X, Agatep R, Chiappa S, Gao L, Lowrance A, Hao A, Goldstein AM, Stavrou T, Scherer SW, Dura WT, Wainwright B, Squire JA, Rutka JT, Hogg D. Mutations in SUFU predispose to medulloblastoma. Nat Genet. 2002 Jul;31(3):306-10. Epub 2002 Jun 17. PMID:12068298 doi:10.1038/ng916
- ↑ Murone M, Luoh SM, Stone D, Li W, Gurney A, Armanini M, Grey C, Rosenthal A, de Sauvage FJ. Gli regulation by the opposing activities of fused and suppressor of fused. Nat Cell Biol. 2000 May;2(5):310-2. PMID:10806483 doi:10.1038/35010610
- ↑ Chi S, Xie G, Liu H, Chen K, Zhang X, Li C, Xie J. Rab23 negatively regulates Gli1 transcriptional factor in a Su(Fu)-dependent manner. Cell Signal. 2012 Jun;24(6):1222-8. doi: 10.1016/j.cellsig.2012.02.004. Epub 2012, Feb 18. PMID:22365972 doi:10.1016/j.cellsig.2012.02.004