2qcx: Difference between revisions
New page: left|200px<br /><applet load="2qcx" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qcx, resolution 2.200Å" /> '''Crystal structure o... |
No edit summary |
||
| Line 2: | Line 2: | ||
caption="2qcx, resolution 2.200Å" /> | caption="2qcx, resolution 2.200Å" /> | ||
'''Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine'''<br /> | '''Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine'''<br /> | ||
==Overview== | |||
TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described. | |||
==About this Structure== | ==About this Structure== | ||
2QCX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=PF1:'>PF1</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Thiaminase Thiaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.2 3.5.99.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QCX OCA]. | 2QCX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=PF1:'>PF1</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Thiaminase Thiaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.2 3.5.99.2] Known structural/functional Sites: <scene name='pdbsite=AC1:Pf1+Binding+Site+For+Residue+A+300'>AC1</scene> and <scene name='pdbsite=AC2:Pf1+Binding+Site+For+Residue+B+300'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QCX OCA]. | ||
==Reference== | |||
Mutagenesis studies on TenA: A thiamin salvage enzyme from Bacillus subtilis., Jenkins AL, Zhang Y, Ealick SE, Begley TP, Bioorg Chem. 2008 Feb;36(1):29-32. Epub 2007 Dec 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18054064 18054064] | |||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thiaminase]] | [[Category: Thiaminase]] | ||
[[Category: Begley, T | [[Category: Begley, T P.]] | ||
[[Category: Ealick, S | [[Category: Ealick, S E.]] | ||
[[Category: Jenkins, A | [[Category: Jenkins, A L.]] | ||
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
[[Category: PF1]] | [[Category: PF1]] | ||
| Line 16: | Line 22: | ||
[[Category: up-down bundle]] | [[Category: up-down bundle]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:38:15 2008'' | ||
Revision as of 19:38, 21 February 2008
|
Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine
OverviewOverview
TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.
About this StructureAbout this Structure
2QCX is a Single protein structure of sequence from Bacillus subtilis with as ligand. Active as Thiaminase, with EC number 3.5.99.2 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Mutagenesis studies on TenA: A thiamin salvage enzyme from Bacillus subtilis., Jenkins AL, Zhang Y, Ealick SE, Begley TP, Bioorg Chem. 2008 Feb;36(1):29-32. Epub 2007 Dec 3. PMID:18054064
Page seeded by OCA on Thu Feb 21 18:38:15 2008