Sandbox 1k4r: Difference between revisions

← Older edit Newer edit →

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Sunjeet Virdi

Revision as of 18:59, 14 November 2013 view source Sunjeet Virdi (talk \| contribs) 59 edits No edit summary ← Older edit		Revision as of 19:01, 14 November 2013 view source Sunjeet Virdi (talk \| contribs) 59 edits No edit summary Newer edit →
Line 26:		Line 26:

	'''Dengue NS3/NS2B Protein'''<StructureSection load='2vbc' size='500' side='right' caption='Structure of protease and helicase (PDB entry [[2vbc]])' scene=''>The NS3 protease is a serine protease that can also function as a RNA helicase and RTPase/NTPase. The enzymatic function of this protease is important for the Dengue virus to replicate. This enzyme of the virus is also a potential target for vaccines and antiviral drugs.		'''Dengue NS3/NS2B Protein'''<StructureSection load='2vbc' size='500' side='right' caption='Structure of protease and helicase (PDB entry [[2vbc]])' scene=''>The NS3 protease is a serine protease that can also function as a RNA helicase and RTPase/NTPase. The enzymatic function of this protease is important for the Dengue virus to replicate. This enzyme of the virus is also a potential target for vaccines and antiviral drugs.
	The catalytic triad <scene name='56/565763/Ns2b/3'>(His-51, Asp-75 and Ser-135)</scene>, is found between these two β-barrels, and its activity is dependent on the presence of the <scene name='56/565763/Ns2b/6'>NS2B</scene>. This cofactor then wraps around the NS3 protease domain and becomes part of the active site. The NS2B cofactor is critical for proteolytic activation of the NS3 protease. The NS3 protease is made up of an extensive network of hydrogen bond and hydrophobic interaction, making it very rigid. NS2B is also important in contributing to substrate binding. This implies that NS2B acts as an enzyme activator as well as being directly involved in substrate binding/interactions.</StructureSection>		The catalytic triad <scene name='56/565763/Ns2b/3'>(His-51, Asp-75 and Ser-135)</scene>, is found between these two β-barrels, and its activity is dependent on the presence of the <scene name='56/565763/Ns2b/7'>NS2B</scene>. This cofactor then wraps around the NS3 protease domain and becomes part of the active site. The NS2B cofactor is critical for proteolytic activation of the NS3 protease. The NS3 protease is made up of an extensive network of hydrogen bond and hydrophobic interaction, making it very rigid. NS2B is also important in contributing to substrate binding. This implies that NS2B acts as an enzyme activator as well as being directly involved in substrate binding/interactions.</StructureSection>