2pxt: Difference between revisions
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==Overview== | ==Overview== | ||
X-ray crystallography of biologically important RNA molecules has been | X-ray crystallography of biologically important RNA molecules has been hampered by technical challenges, including finding heavy-atom derivatives to obtain high-quality experimental phase information. Existing techniques have drawbacks, limiting the rate at which important new structures are solved. To address this, we have developed a reliable means to localize heavy atoms specifically to virtually any RNA. By solving the crystal structures of thirteen variants of the G*U wobble pair cation binding motif, we have identified a version that when inserted into an RNA helix introduces a high-occupancy cation binding site suitable for phasing. This "directed soaking" strategy can be integrated fully into existing RNA crystallography methods, potentially increasing the rate at which important structures are solved and facilitating routine solving of structures using Cu-Kalpha radiation. This method already has been used to solve several crystal structures. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Batey, R | [[Category: Batey, R T.]] | ||
[[Category: Keel, A | [[Category: Keel, A Y.]] | ||
[[Category: Kieft, J | [[Category: Kieft, J S.]] | ||
[[Category: Rambo, R | [[Category: Rambo, R P.]] | ||
[[Category: NCO]] | [[Category: NCO]] | ||
[[Category: cation binding]] | [[Category: cation binding]] | ||
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[[Category: signaling protein/rna complex]] | [[Category: signaling protein/rna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:34:06 2008'' | ||
Revision as of 19:34, 21 February 2008
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Variant 15 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle
OverviewOverview
X-ray crystallography of biologically important RNA molecules has been hampered by technical challenges, including finding heavy-atom derivatives to obtain high-quality experimental phase information. Existing techniques have drawbacks, limiting the rate at which important new structures are solved. To address this, we have developed a reliable means to localize heavy atoms specifically to virtually any RNA. By solving the crystal structures of thirteen variants of the G*U wobble pair cation binding motif, we have identified a version that when inserted into an RNA helix introduces a high-occupancy cation binding site suitable for phasing. This "directed soaking" strategy can be integrated fully into existing RNA crystallography methods, potentially increasing the rate at which important structures are solved and facilitating routine solving of structures using Cu-Kalpha radiation. This method already has been used to solve several crystal structures.
About this StructureAbout this Structure
2PXT is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
A general strategy to solve the phase problem in RNA crystallography., Keel AY, Rambo RP, Batey RT, Kieft JS, Structure. 2007 Jul;15(7):761-72. PMID:17637337
Page seeded by OCA on Thu Feb 21 18:34:06 2008