ATP-dependent DNA ligase from bacteriophage T7: Difference between revisions

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William Guthrie, Jeremy A. Hammett, Hunter Douglas, Jaime Prilusky

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 ==Catalytic function with DNA==
-ATP-dependent DNA ligase from bacteriophage T7 amends a fractured DNA strand through esterification of a 5'- phosphoryl to a 3'- hydroxyl group.  This mechanism occurs with the aid of ATP in several steps.  First, the ligase is activated through a <scene name='56/567310/Amp_complex/1'>phosphoramidate bond with a lysine residue</scene> in the active site (Lys 34).  A pyrophosphate leaves and the enzyme-AMP complex is formed.  Next, the AMP is transferred to the 5' phosphate group at the nick in the DNA.  Finally, T7 ligase creates the phosphodiester bond between the 5' -phosphoryl and the 3' – hydroxyl group, with AMP being freed.  All ATP-dependent DNA ligases contain a conserved amino acid sequence of KxDGxR.  This includes the lysine residue which binds the ATP in the groove between the two domains.
+ATP-dependent DNA ligase from bacteriophage T7 amends a fractured DNA strand through esterification of a 5'- phosphoryl to a 3'- hydroxyl group.  This mechanism occurs with the aid of ATP in several steps.  First, the ligase is activated through a <scene name='56/567310/Amp_complex/1'>phosphoramidate bond with a lysine residue</scene> in the active site (Lys 34).  A pyrophosphate leaves and the enzyme-AMP complex is formed.  Next, the AMP is transferred to the 5' phosphate group at the nick in the DNA.  Finally, T7 ligase creates the phosphodiester bond between the 5' -phosphoryl and the 3' – hydroxyl group, with AMP being freed.  All ATP-dependent DNA ligases contain a conserved amino acid sequence of KxDGxR<ref>PMID: 8626651</ref>.  This includes the lysine residue which binds the ATP in the groove between the two domains.
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