Sandbox 1k4r: Difference between revisions
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Dengue NS5 Protein<StructureSection load='2j7w' size='500' side='right' caption='NS5 polymerase (PDB entry [[2j7w]])' scene=''>The NS5 protein is a 900-residue peptide, which contains a methyltransferase domain. This protein plays an important role in the Dengue virus replication. The protein not only functions as a methyltransferase, but also as a RNA polymerase. The NS5 protein also contains guanylyltransferase activities, which, along with methyltransferase, help protect the viral genome and create efficient protein translation. | Dengue NS5 Protein<StructureSection load='2j7w' size='500' side='right' caption='NS5 polymerase (PDB entry [[2j7w]])' scene=''>The NS5 protein is a 900-residue peptide, which contains a methyltransferase domain. This protein plays an important role in the Dengue virus replication. The protein not only functions as a methyltransferase, but also as a RNA polymerase. The NS5 protein also contains guanylyltransferase activities, which, along with methyltransferase, help protect the viral genome and create efficient protein translation. | ||
There are four serotypes of the Dengue virus, and the NS5 protein is most prominent in the Dengue-2-serotype, helping it with its pathogenesis.<scene name='56/565763/ | There are four serotypes of the Dengue virus, and the NS5 protein is most prominent in the Dengue-2-serotype, helping it with its pathogenesis. | ||
Dengue-2 NS5 contains an also has an <scene name='56/565763/Sah/4'>active site</scene> which is considered a "S-adenosyl methionine-dependent methyltransferase fold" structure. The S-adenosyl methionine ligand is methylated in the methyltransferase domain, creating S-adenosyl-l-homocysteine (<scene name='56/565763/Sah/5'>SAH</scene>)as a by-product. | |||
In the NS5 protein, there is another binding site for GTP. This binding of GTP is done in the N-terminal domain on the protein. The <scene name='56/565763/Active_site_gtp/3'>GTP in the binding site</scene> is to be a cap-binding site for the Dengue-2-methyltransferase. Overall the GTP is required for translation, transcription and replication processes.</StructureSection> | |||
Dengue NS3/NS2B Protein<StructureSection load='2vbc' size='500' side='right' caption='Structure of protease and helicase (PDB entry [[2vbc]])' scene=''>The NS3 protease is a serine protease that can also function as a RNA helicase and RTPase/NTPase. The enzymatic function of this protease is important for the Dengue virus to replicate. This enzyme of the virus is also a potential target for vaccines and antiviral drugs. | Dengue NS3/NS2B Protein<StructureSection load='2vbc' size='500' side='right' caption='Structure of protease and helicase (PDB entry [[2vbc]])' scene=''>The NS3 protease is a serine protease that can also function as a RNA helicase and RTPase/NTPase. The enzymatic function of this protease is important for the Dengue virus to replicate. This enzyme of the virus is also a potential target for vaccines and antiviral drugs. | ||