Single stranded binding protein: Difference between revisions

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==Introduction==
==Introduction==
The single-stranded DNA-binding protein (SSB) of Escherichia coli is involved in all aspects of DNA metabolism: replication, repair, and recombination.
In solution, the protein exists as a homotetramer of 18,843-kilodalton subunits. As it binds tightly and cooperatively to single-stranded DNA, it has become
a prototypic model protein for studying protein-nucleic acid interactions. The sequences of the gene and protein are known, and the functional domains of
subunit interaction, DNA binding, and protein-protein interactions have been probed by structure-function analyses of various mutations. The ssb gene has
three promoters, one of which is inducible because it lies only two nucleotides from the LexA-binding site of the adjacent uvrA gene. Induction of the SOS
response, however, does not lead to significant increases in SSB levels. The binding protein has several functions in DNA replication, including enhancement
of helix destabilization by DNA helicases, prevention of reannealing of the single strands and protection from nuclease digestion, organization and stabilization
of replication origins, primosome assembly, priming specificity, enhancement of replication fidelity, enhancement of polymerase processivity, and promotion
of polymerase binding to the template. E. coli SSB is required for methyl-directed mismatch repair, induction of the SOS response, and recombinational repair.
During recombination, SSB interacts with the RecBCD enzyme to find Chi sites, promotes binding of RecA protein, and promotes strand uptake.


Copies of SSB bind to the unwound DNA strands, keeping the strands separated so that both strands can serve as templates.  
Copies of SSB bind to the unwound DNA strands, keeping the strands separated so that both strands can serve as templates.  

Revision as of 18:55, 30 October 2013

Sandbox Single Stranded DNA-Binding Protein (SSB)Sandbox Single Stranded DNA-Binding Protein (SSB)


Introduction

Copies of SSB bind to the unwound DNA strands, keeping the strands separated so that both strands can serve as templates.

Structure

The structure of SSB consists of a homotetramer that has a DNA binding domain that binds to a single strand of DNA.

Binding Interactions in the Active Site

See Also

References


PMID: 2087220


Structure of Single Stranded DNA-Binding Protein bound to ssDNA (PDB entry 1eyg)

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Refayat Ahsen, Rachel Craig, Alexander Berchansky, Michal Harel
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