2pkv: Difference between revisions
New page: left|200px<br /><applet load="2pkv" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pkv, resolution 1.60Å" /> '''D-(GGTATACC) ambient... |
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==Overview== | ==Overview== | ||
The behaviour of the d(GGTATACC) oligonucleotide has been investigated by | The behaviour of the d(GGTATACC) oligonucleotide has been investigated by X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa (approximately 20,000 atm). Four 3D-structures of the A-DNA form (at ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A resolution. In addition to the diffraction pattern of the A-form, the broad meridional streaks previously explained by occluded B-DNA octamers within the channels of the crystalline A-form matrix were observed up to at least 2 GPa. This work highlights an important property of nucleic acids, their capability to withstand very high pressures, while keeping in such conditions a nearly invariant geometry of base pairs that store and carry genetic information. The double-helix base-paired architecture behaves as a molecular spring, which makes it especially adapted to very harsh conditions. These features may have contributed to the emergence of a RNA World at prebiotic stage. | ||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007 Jul 7 | Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007;35(14):4800-8. Epub 2007 Jul 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17617642 17617642] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Fourme, R.]] | [[Category: Fourme, R.]] | ||
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[[Category: high pressure]] | [[Category: high pressure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:30:29 2008'' | ||
Revision as of 19:30, 21 February 2008
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D-(GGTATACC) ambient pressure
OverviewOverview
The behaviour of the d(GGTATACC) oligonucleotide has been investigated by X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa (approximately 20,000 atm). Four 3D-structures of the A-DNA form (at ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A resolution. In addition to the diffraction pattern of the A-form, the broad meridional streaks previously explained by occluded B-DNA octamers within the channels of the crystalline A-form matrix were observed up to at least 2 GPa. This work highlights an important property of nucleic acids, their capability to withstand very high pressures, while keeping in such conditions a nearly invariant geometry of base pairs that store and carry genetic information. The double-helix base-paired architecture behaves as a molecular spring, which makes it especially adapted to very harsh conditions. These features may have contributed to the emergence of a RNA World at prebiotic stage.
About this StructureAbout this Structure
2PKV is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007;35(14):4800-8. Epub 2007 Jul 7. PMID:17617642
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