2pa8: Difference between revisions
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[[Image:2pa8.jpg|left|200px]]<br /><applet load="2pa8" size="350" color="white" frame="true" align="right" spinBox="true" | [[Image:2pa8.jpg|left|200px]]<br /><applet load="2pa8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2pa8, resolution 1.760Å" /> | caption="2pa8, resolution 1.760Å" /> | ||
'''X-Ray Crystal Structure of | '''X-Ray Crystal Structure of the D/L Subcomplex of the Sulfolobus Solfataricus RNA polymerase'''<br /> | ||
==Overview== | |||
The transcription apparatus in Archaea can be described as a simplified version of its eukaryotic RNA polymerase (RNAP) II counterpart, comprising an RNAPII-like enzyme as well as two general transcription factors, the TATA-binding protein (TBP) and the eukaryotic TFIIB orthologue TFB. It has been widely understood that precise comparisons of cellular RNAP crystal structures could reveal structural elements common to all enzymes and that these insights would be useful in analysing components of each enzyme that enable it to perform domain-specific gene expression. However, the structure of archaeal RNAP has been limited to individual subunits. Here we report the first crystal structure of the archaeal RNAP from Sulfolobus solfataricus at 3.4 A resolution, completing the suite of multi-subunit RNAP structures from all three domains of life. We also report the high-resolution (at 1.76 A) crystal structure of the D/L subcomplex of archaeal RNAP and provide the first experimental evidence of any RNAP possessing an iron-sulphur (Fe-S) cluster, which may play a structural role in a key subunit of RNAP assembly. The striking structural similarity between archaeal RNAP and eukaryotic RNAPII highlights the simpler archaeal RNAP as an ideal model system for dissecting the molecular basis of eukaryotic transcription. | |||
==About this Structure== | ==About this Structure== | ||
2PA8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=F3S:'>F3S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Known structural/functional Sites: <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+D+400'>AC1</scene> and <scene name='pdbsite=AC2:F3s+Binding+Site+For+Residue+D+3076'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PA8 OCA]. | 2PA8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=F3S:'>F3S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Known structural/functional Sites: <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+D+400'>AC1</scene> and <scene name='pdbsite=AC2:F3s+Binding+Site+For+Residue+D+3076'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PA8 OCA]. | ||
==Reference== | |||
The X-ray crystal structure of RNA polymerase from Archaea., Hirata A, Klein BJ, Murakami KS, Nature. 2008 Feb 14;451(7180):851-4. Epub 2008 Jan 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18235446 18235446] | |||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Sulfolobus solfataricus]] | [[Category: Sulfolobus solfataricus]] | ||
[[Category: Hirata, A.]] | [[Category: Hirata, A.]] | ||
[[Category: Murakami, K | [[Category: Murakami, K S.]] | ||
[[Category: F3S]] | [[Category: F3S]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:27:38 2008'' | ||