2p6b: Difference between revisions
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==Overview== | ==Overview== | ||
The protein phosphatase calcineurin recognizes a wide assortment of | The protein phosphatase calcineurin recognizes a wide assortment of substrates and controls diverse developmental and physiological pathways in eukaryotic cells. Dephosphorylation of the transcription factor NFAT and certain other calcineurin substrates depends on docking of calcineurin at a PxIxIT consensus site. We describe here the structural basis for recognition of the PxIxIT sequence by calcineurin. We demonstrate that the high-affinity peptide ligand PVIVIT adds as a beta-strand to the edge of a beta-sheet of calcineurin; that short peptide segments containing the PxIxIT consensus sequence suffice for calcineurin-substrate docking; and that sequence variations within the PxIxIT core modulate the K(d) of the interaction within the physiological range 1 microM to 1 mM. Calcineurin can adapt to a wide variety of substrates, because recognition requires only a PxIxIT sequence and because variation within the core PxIxIT sequence can fine-tune the affinity to match the physiological signalling requirements of individual substrates. | ||
==Disease== | |||
Known diseases associated with this structure: Cornea plana congenita, recessive OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=603288 603288]], Myotonic dystrophy, type 2 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=116955 116955]] | |||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
Structure of | Structure of calcineurin in complex with PVIVIT peptide: portrait of a low-affinity signalling interaction., Li H, Zhang L, Rao A, Harrison SC, Hogan PG, J Mol Biol. 2007 Jun 22;369(5):1296-306. Epub 2007 Apr 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17498738 17498738] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Harrison, S | [[Category: Harrison, S C.]] | ||
[[Category: Hogan, P | [[Category: Hogan, P G.]] | ||
[[Category: Li, H.]] | [[Category: Li, H.]] | ||
[[Category: Rao, A.]] | [[Category: Rao, A.]] | ||
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[[Category: beta-sheet augmentation; protein-peptide complex]] | [[Category: beta-sheet augmentation; protein-peptide complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:26:17 2008'' | ||
Revision as of 19:26, 21 February 2008
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Crystal Structure of Human Calcineurin in Complex with PVIVIT Peptide
OverviewOverview
The protein phosphatase calcineurin recognizes a wide assortment of substrates and controls diverse developmental and physiological pathways in eukaryotic cells. Dephosphorylation of the transcription factor NFAT and certain other calcineurin substrates depends on docking of calcineurin at a PxIxIT consensus site. We describe here the structural basis for recognition of the PxIxIT sequence by calcineurin. We demonstrate that the high-affinity peptide ligand PVIVIT adds as a beta-strand to the edge of a beta-sheet of calcineurin; that short peptide segments containing the PxIxIT consensus sequence suffice for calcineurin-substrate docking; and that sequence variations within the PxIxIT core modulate the K(d) of the interaction within the physiological range 1 microM to 1 mM. Calcineurin can adapt to a wide variety of substrates, because recognition requires only a PxIxIT sequence and because variation within the core PxIxIT sequence can fine-tune the affinity to match the physiological signalling requirements of individual substrates.
DiseaseDisease
Known diseases associated with this structure: Cornea plana congenita, recessive OMIM:[603288], Myotonic dystrophy, type 2 OMIM:[116955]
About this StructureAbout this Structure
2P6B is a Protein complex structure of sequences from Homo sapiens with , , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structure of calcineurin in complex with PVIVIT peptide: portrait of a low-affinity signalling interaction., Li H, Zhang L, Rao A, Harrison SC, Hogan PG, J Mol Biol. 2007 Jun 22;369(5):1296-306. Epub 2007 Apr 19. PMID:17498738
Page seeded by OCA on Thu Feb 21 18:26:17 2008