15.5kD/Snu13/L7Ae protein: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Kelly Hrywkiw, Alexander Berchansky, Wayne Decatur

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 == Structure of the protein homologues: 15.5kD, Snu13, and L7Ae ==
-by Kelly Hrywkiw
 __TOC__
 =Introduction=
-The human protein 15.5kD and its yeast (Snu13p) and archaeal (L7Ae) homologues function in the processing of pre-ribosomal RNA as part of the box [http://en.wikipedia.org/wiki/Small_nucleolar_RNA#C.2FD_box C/D] and [http://en.wikipedia.org/wiki/Small_nucleolar_RNA#H.2FACA_box H/ACA] small ribonucleoprotein particle (sRNP – [http://en.wikipedia.org/wiki/Archaea archaea]) or small nucleolar ribonucleoprotein particle (snoRNP – [http://en.wikipedia.org/wiki/Eukarya eukarya]) nucleotide modification complexes  (s(no)RNPs)<ref name ="gagnon">PMCID:PMC2802039</ref><ref name ="dobbyn">PMID:17631273</ref>.  In addition, 15.5kD and Snu13p function in U4 [http://en.wikipedia.org/wiki/SnRNP small nuclear ribonucleoprotein particle] (snRNP) spliceosomal biogenesis<ref name ="dobbyn"/>.  The capability to function in dual roles lies in the ability to recognize a helix-bulge-helix (kink-turn) RNA motif that is present in the different RNPs<ref name ="oruganti">PMID:15963469</ref>
+The human protein 15.5kD and its yeast (Snu13p) and archaeal (L7Ae) homologues function in the processing of pre-ribosomal RNA as part of the box [http://en.wikipedia.org/wiki/Small_nucleolar_RNA#C.2FD_box C/D] and [http://en.wikipedia.org/wiki/Small_nucleolar_RNA#H.2FACA_box H/ACA] small ribonucleoprotein particle (sRNP – [http://en.wikipedia.org/wiki/Archaea archaea]) or small nucleolar ribonucleoprotein particle (snoRNP – [http://en.wikipedia.org/wiki/Eukarya eukarya]) nucleotide modification complexes  (s(no)RNPs)<ref name ="gagnon">PMCID:PMC2802039</ref><ref name ="dobbyn">PMID:17631273</ref>.  In addition, 15.5kD and Snu13p function in U4 [http://en.wikipedia.org/wiki/SnRNP small nuclear ribonucleoprotein particle] (snRNP) spliceosomal biogenesis<ref name ="dobbyn"/>.  The capability to function in dual roles lies in the ability to recognize a [[Kink-turn motif|helix-bulge-helix (kink-turn) RNA motif]] that is present in the different RNPs<ref name ="oruganti">PMID:15963469</ref>
-A variation of the kink-turn motif, known as the kink-loop motif, can be found in the C/D and H/ACA RNAs <ref name ="gagnon"/>.  Interestingly, the eukaryotic proteins and their archaeal homologue do not interact with the different motifs in the same manner, even though share a conserved sequence similarity <ref name ="oruganti"/>.  For example, while L7Ae exhibits the same binding affinity for both the kink-turn and kink-loop sRNA motifs, its eukaryotic homologues only bind specifically to the kink-turn motif and discriminate against the kink-loop motif <ref name ="oruganti"/><ref name ="gagnon"/>.
+A variation of the [[Kink-turn motif|kink-turn motif]], known as the kink-loop motif, can be found in the C/D and H/ACA RNAs <ref name ="gagnon"/>.  Interestingly, the eukaryotic proteins and their archaeal homologue do not interact with the different motifs in the same manner, even though share a conserved sequence similarity <ref name ="oruganti"/>.  For example, while L7Ae exhibits the same binding affinity for both the [[Kink-turn motif|kink-turn]] and kink-loop sRNA motifs, its eukaryotic homologues only bind specifically to the [[Kink-turn motif|kink-turn motif]] and discriminate against the kink-loop motif <ref name ="oruganti"/><ref name ="gagnon"/>.
 Solved structures of the proteins include: 15.5kD in complex with a U4 snRNA fragment [[1E7K]], 15.5kD in complex with hPrp31 and a U4 snRNA fragment [[2OZB]], Snu13p without RNA [[1ZWZ]], [http://en.wikipedia.org/wiki/Archaeoglobus Archaeoglobus fulgidus] L7Ae-box C/D with RNA [[1RLG]], [http://en.wikipedia.org/wiki/Methanococcus_jannaschii Methanococcus jannaschii] L7Ae-H/ACA with RNA [[1RA4]], and [http://en.wikipedia.org/wiki/Pyrococcus Pyrococcus abyssi] L7Ae without RNA [[1PXW]].