User:Alice Harmon/Sandbox 5: Difference between revisions

Line 18:

{|

|'''Left ~~scene~~''' - apo PYL2 [[3kdh]]

|'''Left panel''' - apo PYL2 [[3kdh]]

|'''Middle ~~scene~~''' - ABA bound to PYL2 [[3kdi]]

|'''Middle panel''' - ABA bound to PYL2 [[3kdi]] or PYR1 [[3k3k]]

|'''Right ~~scene~~''' - PYL.ABA bound to HAB1[[3ujl]]

|'''Right panel''' - PYL'''.'''ABA bound to HAB1[[3ujl]]

|-

| <applet load='3kdh' size='300' frame='true' align='left' caption='3kaz - apo-Pyl2' scene = '56/564059/Apopyl2/1'/> '''3kdh scenes''' <scene name='56/564059/Apopyl2/1'>1. Default scene </scene> <scene name='56/564059/Apopyl2/3'>2. Open gate</scene>

| <applet load='3kdh' size='300' frame='true' align='left' caption='3kaz - apo-Pyl2' scene = '56/564059/Apopyl2/1'/> '''3kdh scenes''' <scene name='56/564059/Apopyl2/1'>1. Default scene </scene> PYL2 is shown as a monomer. See also Scene 3 of the middle panel, which shows the receptor dimer. <scene name='56/564059/Apopyl2/3'>2. Open gate</scene> The entrance to binding pocket for ABA is regulated by a "latch" shown in orchid and a "gate" shown in blue. Proline 92 is shown in ball and stick. Here the gate and entrance to the binding site are open.

| <applet load='3kdi' size='300' frame='true' align='left' caption='3kdi - ABA bound to PYL2' scene = '56/564059/Abapyl2/1' /> '''3ujg scenes''' <scene name='56/564059/Abapyl2/1'>1. Default scene </scene> <scene name='56/564059/Abapyl2/2'>2. Closed gate </scene><scene name='56/564059/Pyr1dimer/2'>PYR1 dimer</scene> in which one monomer is bound to ABA.

| <applet load='3kdi' size='300' frame='true' align='left' caption='3kdi - ABA bound to PYL2' scene = '56/564059/Abapyl2/1' /> '''3ujg scenes''' <scene name='56/564059/Abapyl2/1'>1. Default scene </scene> ABA (CPK spheres) binds to a water-filled (not shown) pocket of PYL2. <scene name='56/564059/Abapyl2/2'>2. Closed gate </scene> The gate folds over ABA and interacts with the latch. <scene name='56/564059/Pyr1dimer/2'>3. PYR1 dimer</scene> in which one monomer is bound to ABA. The native form of the receptor is a dimer<ref>PMID:19933100</ref><ref>PMID:23265948</ref>.

|<applet load='3ujl' size='300' frame='true' align='left' caption='3ujl - PYR2-HAB1' scene = '56/564059/Pyl2hab1/2' /> '''3ujg scenes''' <scene name='56/564059/Pyl2hab1/1'>~~TextToBeDisplayed~~</scene> <scene name='56/564059/Pyl2hab1/4'>2. ~~Gate~~ interaction with HAB1</scene>

|<applet load='3ujl' size='300' frame='true' align='left' caption='3ujl - PYR2-HAB1' scene = '56/564059/Pyl2hab1/2' /> '''3ujg scenes''' <scene name='56/564059/Pyl2hab1/1'>1. Default scene</scene> Complex between PYL2 (blue) with bound ABA (CPK spheres)and HAB1 (gold), a protein phosphatase 2C. Magnesium ions in the active site of HAB1 are shown as green spheres. <scene name='56/564059/Pyl2hab1/5'>2. Closed gate locked by interaction with HAB1</scene> Gate residue proline 92 (blue ball and stick) interacts with typtophan 290 (gold ball and stick and residues in a hydrophobic loop (dark gold ball and stick) of HAB1. The gate also interacts with residues surrounding the phosphatase's active site, which is marked by magnesium ions (small green spheres).

|}

@@ Line 18: / Line 18: @@
 {|
-|'''Left scene''' - apo PYL2 [[3kdh]]
+|'''Left panel''' - apo PYL2 [[3kdh]]
-|'''Middle scene''' - ABA bound to PYL2 [[3kdi]]
+|'''Middle panel''' - ABA bound to PYL2 [[3kdi]] or PYR1 [[3k3k]]
-|'''Right scene''' - PYL.ABA bound to HAB1[[3ujl]]
+|'''Right panel''' - PYL<sup>'''.'''</sup>ABA bound to HAB1[[3ujl]]
 |-
-| <applet load='3kdh' size='300' frame='true' align='left' caption='3kaz - apo-Pyl2' scene = '56/564059/Apopyl2/1'/><br  clear='both'>'''3kdh scenes''' <Br><scene name='56/564059/Apopyl2/1'>1. Default scene </scene><br><scene name='56/564059/Apopyl2/3'>2. Open gate</scene>
+| <applet load='3kdh' size='300' frame='true' align='left' caption='3kaz - apo-Pyl2' scene = '56/564059/Apopyl2/1'/><br  clear='both'>'''3kdh scenes''' <Br><scene name='56/564059/Apopyl2/1'>1. Default scene </scene> PYL2 is shown as a monomer. See also Scene 3 of the middle panel, which shows the receptor dimer. <br><scene name='56/564059/Apopyl2/3'>2. Open gate</scene> The entrance to binding pocket for ABA is regulated by a "latch" shown in orchid and a "gate" shown in blue. Proline 92 is shown in ball and stick. Here the gate and entrance to the binding site are open.
-| <applet load='3kdi' size='300' frame='true' align='left' caption='3kdi - ABA bound to PYL2' scene = '56/564059/Abapyl2/1' /><Br clear='both'>'''3ujg scenes'''<Br><scene name='56/564059/Abapyl2/1'>1. Default scene </scene><br><scene name='56/564059/Abapyl2/2'>2. Closed gate </scene><scene name='56/564059/Pyr1dimer/2'>PYR1 dimer</scene> in which one monomer is bound to ABA.
+| <applet load='3kdi' size='300' frame='true' align='left' caption='3kdi - ABA bound to PYL2' scene = '56/564059/Abapyl2/1' /><Br clear='both'>'''3ujg scenes'''<Br><scene name='56/564059/Abapyl2/1'>1. Default scene </scene> ABA (CPK spheres) binds to a water-filled (not shown) pocket of PYL2. <br><scene name='56/564059/Abapyl2/2'>2. Closed gate </scene> The gate folds over ABA and interacts with the latch. <br><scene name='56/564059/Pyr1dimer/2'>3. PYR1 dimer</scene> in which one monomer is bound to ABA. The native form of the receptor is a dimer<ref>PMID:19933100</ref><ref>PMID:23265948</ref>.
-|<applet load='3ujl' size='300' frame='true' align='left' caption='3ujl - PYR2-HAB1' scene = '56/564059/Pyl2hab1/2' /><Br clear='both'>'''3ujg scenes'''<Br><scene name='56/564059/Pyl2hab1/1'>TextToBeDisplayed</scene><br><scene name='56/564059/Pyl2hab1/4'>2. Gate interaction with HAB1</scene>
+|<applet load='3ujl' size='300' frame='true' align='left' caption='3ujl - PYR2-HAB1' scene = '56/564059/Pyl2hab1/2' /><Br clear='both'>'''3ujg scenes'''<Br><scene name='56/564059/Pyl2hab1/1'>1. Default scene</scene> Complex between PYL2 (blue) with bound ABA (CPK spheres)and HAB1 (gold), a protein phosphatase 2C. Magnesium ions in the active site of HAB1 are shown as green spheres. <br><scene name='56/564059/Pyl2hab1/5'>2. Closed gate locked by interaction with HAB1</scene> Gate residue proline 92 (blue ball and stick) interacts with typtophan 290 (gold ball and stick and residues in a hydrophobic loop (dark gold ball and stick) of HAB1. The gate also interacts with residues surrounding the phosphatase's active site, which is marked by magnesium ions (small green spheres).
 |}