2olw: Difference between revisions
New page: left|200px<br /><applet load="2olw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2olw, resolution 1.600Å" /> '''Crystal Structure o... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2olw.gif|left|200px]]<br /><applet load="2olw" size=" | [[Image:2olw.gif|left|200px]]<br /><applet load="2olw" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2olw, resolution 1.600Å" /> | caption="2olw, resolution 1.600Å" /> | ||
'''Crystal Structure of E. coli pseudouridine synthase RluE'''<br /> | '''Crystal Structure of E. coli pseudouridine synthase RluE'''<br /> | ||
==Overview== | ==Overview== | ||
Pseudouridine synthase RluE modifies U2457 in a stem of 23 S RNA in | Pseudouridine synthase RluE modifies U2457 in a stem of 23 S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome. We determined the crystal structures of the C-terminal, catalytic domain of E. coli RluE at 1.2 A resolution and of full-length RluE at 1.6 A resolution. The crystals of the full-length enzyme contain two molecules in the asymmetric unit and in both molecules the N-terminal domain is disordered. The protein has an active site cleft, conserved in all other pseudouridine synthases, that contains invariant Asp and Tyr residues implicated in catalysis. An electropositive surface patch that covers the active site cleft is just wide enough to accommodate an RNA stem. The RNA substrate stem can be docked to this surface such that the catalytic Asp is adjacent to the target base, and a conserved Arg is positioned to help flip the target base out of the stem into the enzyme active site. A flexible RluE specific loop lies close to the conserved region of the stem in the model, and may contribute to substrate specificity. The stem alone is not a good RluE substrate, suggesting RluE makes additional interactions with other regions in the ribosome. | ||
==About this Structure== | ==About this Structure== | ||
2OLW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, DTT and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2OLW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=DTT:'>DTT</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OLW OCA]. | ||
==Reference== | ==Reference== | ||
The | The crystal structure of E. coli rRNA pseudouridine synthase RluE., Pan H, Ho JD, Stroud RM, Finer-Moore J, J Mol Biol. 2007 Apr 13;367(5):1459-70. Epub 2007 Feb 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17320904 17320904] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Finer-Moore, J.]] | [[Category: Finer-Moore, J.]] | ||
[[Category: Ho, J | [[Category: Ho, J D.]] | ||
[[Category: Pan, H.]] | [[Category: Pan, H.]] | ||
[[Category: Stroud, R | [[Category: Stroud, R M.]] | ||
[[Category: ACY]] | [[Category: ACY]] | ||
[[Category: DTT]] | [[Category: DTT]] | ||
| Line 22: | Line 22: | ||
[[Category: bifurcated beta sheet]] | [[Category: bifurcated beta sheet]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:56 2008'' | ||
Revision as of 19:19, 21 February 2008
|
Crystal Structure of E. coli pseudouridine synthase RluE
OverviewOverview
Pseudouridine synthase RluE modifies U2457 in a stem of 23 S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome. We determined the crystal structures of the C-terminal, catalytic domain of E. coli RluE at 1.2 A resolution and of full-length RluE at 1.6 A resolution. The crystals of the full-length enzyme contain two molecules in the asymmetric unit and in both molecules the N-terminal domain is disordered. The protein has an active site cleft, conserved in all other pseudouridine synthases, that contains invariant Asp and Tyr residues implicated in catalysis. An electropositive surface patch that covers the active site cleft is just wide enough to accommodate an RNA stem. The RNA substrate stem can be docked to this surface such that the catalytic Asp is adjacent to the target base, and a conserved Arg is positioned to help flip the target base out of the stem into the enzyme active site. A flexible RluE specific loop lies close to the conserved region of the stem in the model, and may contribute to substrate specificity. The stem alone is not a good RluE substrate, suggesting RluE makes additional interactions with other regions in the ribosome.
About this StructureAbout this Structure
2OLW is a Single protein structure of sequence from Escherichia coli with , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of E. coli rRNA pseudouridine synthase RluE., Pan H, Ho JD, Stroud RM, Finer-Moore J, J Mol Biol. 2007 Apr 13;367(5):1459-70. Epub 2007 Feb 7. PMID:17320904
Page seeded by OCA on Thu Feb 21 18:19:56 2008