2oh6: Difference between revisions

Revision as of 19:18, 21 February 2008

The Crystal Structure of Recombinant Cypovirus Polyhedra

OverviewOverview

Cypoviruses and baculoviruses are notoriously difficult to eradicate because the virus particles are embedded in micrometre-sized protein crystals called polyhedra. The remarkable stability of polyhedra means that, like bacterial spores, these insect viruses remain infectious for years in soil. The environmental persistence of polyhedra is the cause of significant losses in silkworm cocoon harvests but has also been exploited against pests in biological alternatives to chemical insecticides. Although polyhedra have been extensively characterized since the early 1900s, their atomic organization remains elusive. Here we describe the 2 A crystal structure of both recombinant and infectious silkworm cypovirus polyhedra determined using crystals 5-12 micrometres in diameter purified from insect cells. These are the smallest crystals yet used for de novo X-ray protein structure determination. We found that polyhedra are made of trimers of the viral polyhedrin protein and contain nucleotides. Although the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into three-dimensional cubic crystals rather than icosahedral shells. The polyhedrin trimers are extensively cross-linked in polyhedra by non-covalent interactions and pack with an exquisite molecular complementarity similar to that of antigen-antibody complexes. The resulting ultrastable and sealed crystals shield the virus particles from environmental damage. The structure suggests that polyhedra can serve as the basis for the development of robust and versatile nanoparticles for biotechnological applications such as microarrays and biopesticides.

About this StructureAbout this Structure

2OH6 is a Single protein structure of sequence from Bombyx mori cypovirus 1 with , , , and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

The molecular organization of cypovirus polyhedra., Coulibaly F, Chiu E, Ikeda K, Gutmann S, Haebel PW, Schulze-Briese C, Mori H, Metcalf P, Nature. 2007 Mar 1;446(7131):97-101. PMID:17330045

Page seeded by OCA on Thu Feb 21 18:18:28 2008

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 ==Overview==
-Cypoviruses and baculoviruses are notoriously difficult to eradicate, because the virus particles are embedded in micrometre-sized protein, crystals called polyhedra. The remarkable stability of polyhedra means, that, like bacterial spores, these insect viruses remain infectious for, years in soil. The environmental persistence of polyhedra is the cause of, significant losses in silkworm cocoon harvests but has also been exploited, against pests in biological alternatives to chemical insecticides., Although polyhedra have been extensively characterized since the early, 1900s, their atomic organization remains elusive. Here we describe the 2 A, crystal structure of both recombinant and infectious silkworm cypovirus, polyhedra determined using crystals 5-12 micrometres in diameter purified, from insect cells. These are the smallest crystals yet used for de novo, X-ray protein structure determination. We found that polyhedra are made of, trimers of the viral polyhedrin protein and contain nucleotides. Although, the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into, three-dimensional cubic crystals rather than icosahedral shells. The, polyhedrin trimers are extensively cross-linked in polyhedra by, non-covalent interactions and pack with an exquisite molecular, complementarity similar to that of antigen-antibody complexes. The, resulting ultrastable and sealed crystals shield the virus particles from, environmental damage. The structure suggests that polyhedra can serve as, the basis for the development of robust and versatile nanoparticles for, biotechnological applications such as microarrays and biopesticides.
+Cypoviruses and baculoviruses are notoriously difficult to eradicate because the virus particles are embedded in micrometre-sized protein crystals called polyhedra. The remarkable stability of polyhedra means that, like bacterial spores, these insect viruses remain infectious for years in soil. The environmental persistence of polyhedra is the cause of significant losses in silkworm cocoon harvests but has also been exploited against pests in biological alternatives to chemical insecticides. Although polyhedra have been extensively characterized since the early 1900s, their atomic organization remains elusive. Here we describe the 2 A crystal structure of both recombinant and infectious silkworm cypovirus polyhedra determined using crystals 5-12 micrometres in diameter purified from insect cells. These are the smallest crystals yet used for de novo X-ray protein structure determination. We found that polyhedra are made of trimers of the viral polyhedrin protein and contain nucleotides. Although the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into three-dimensional cubic crystals rather than icosahedral shells. The polyhedrin trimers are extensively cross-linked in polyhedra by non-covalent interactions and pack with an exquisite molecular complementarity similar to that of antigen-antibody complexes. The resulting ultrastable and sealed crystals shield the virus particles from environmental damage. The structure suggests that polyhedra can serve as the basis for the development of robust and versatile nanoparticles for biotechnological applications such as microarrays and biopesticides.
 ==About this Structure==
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 [[Category: Coulibaly, F.]]
 [[Category: Gutmann, S.]]
-[[Category: Haebel, P.W.]]
+[[Category: Haebel, P W.]]
 [[Category: Ikeda, K.]]
 [[Category: Metcalf, P.]]
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 [[Category: nucleotide binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:11:11 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:18:28 2008''