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User:Alice Harmon/Sandbox 4: Difference between revisions

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<Structure load='3UC4' size='400' frame='true' align='left' caption='3uc4 - apo SnRK2.6' scene='55/559985/Aposnrk2_6/3' />
<Structure load='3UC4' size='400' frame='true' align='left' caption='3uc4 - apo SnRK2.6' scene='55/559985/Aposnrk2_6/3' />


<scene name='55/559985/Aposnrk2_6/4'>Cysteines in transparent kinase</scene>
SNRK2 box is a C-terminal motif that is common to all SNRK2s. It forms a helix that parallels subdomain III.
 
<scene name='55/559985/Aposnrk2_6/5'>Cysteines in space fill kinase</scene>


<scene name='55/559985/Aposnrk2_6critical/1'>Important structures</scene><br>
<scene name='55/559985/Aposnrk2_6critical/1'>Important structures</scene><br>
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<Structure load='3UJG' size='400' frame='true' align='right' caption='3ujg - SnRK2.6 in complex with HAB1' scene='55/559985/Aposnrk2_6/2' />
<Structure load='3UJG' size='400' frame='true' align='right' caption='3ujg - SnRK2.6 in complex with HAB1' scene='55/559985/Aposnrk2_6/2' />
The SnRK2.6–HAB1 complex was constructed as a fusion protein with a H6-tag (MAHHHHHHA) at the N-terminus of SnRK2.6 (residues 11–362) fused to HAB1(172–511) with a GSGSAGSAAGS linker.


<scene name='55/559985/Ost1hab1/1'>Cysteines in the complex</scene>
D296A and E297A surface entropy reduction mutation sites


ABA box (C-terminal acidic domain) not resolved
ABA box is acidic sequence in C-terminus that is common to SNRK2s that are regulated by ABA. This sequence binds to PP2C in biochemical assays, but is not seen in the crystal structure.


<scene name='55/559985/Ost1hab1_interaction/1'>interaction zone</scene><br>
<scene name='55/559985/Ost1hab1_interaction/1'>interaction zone</scene><br>
kinase activation loop in blue<br>
kinase activation loop in blue<br>
phosphatase W385 in brown
phosphatase W385 in brown
unused: <scene name='55/559985/Aposnrk2_6/4'>Cysteines in transparent kinase</scene><br>
<scene name='55/559985/Aposnrk2_6/5'>Cysteines in space fill kinase</scene><br>
<scene name='55/559985/Ost1hab1/1'>Cysteines in the complex</scene>
===SNRK2 structures===
[[3uc3]] ''Arabidopsis thaliana'' SNRK2.3 + Co<sup>2+</sup><br />
[[3zut]] AtSNRK2.6 (D160A mutant)+ ANP<br />
[[3zuu]] AtSNRK2.6 (D160A, S175D mutant) + gold<br />
[[3uc4]] apoAtSNRK2.6 (D59A, E60A mutant)<br />
[[3udb]] apoAtSNRK2.6 (C131A, C157A, C159A, S7A, s29A, s43A, S166A, T175A) <br />
'''complex with a protein phosphatase 2C'''<br />
[[3ujg]] AtSNRK2.6 (D296A)  + HAB1  + Mg<sup>2+</sup>
===PP2C structures===
[[1a6q]] – hPP2C <br />
[[2iq1]] – hPP2C κ <br />
[[2p8e]] – hPP2C β<br />
[[2cm1]] – PP2C + Mn – Micobacterium tuberculosis<br />
[[3d8k]] – PP2C – Toxoplasma gondii<br />
[[3jrq]], [[3kdj]], [[3nmn]] – AtPP2C + Pyl1 – Arabidopsis thaliana<br />
[[3nmt]], [[3kb3]], [[3nmv]], [[3ujl]] – AtPP2C + Pyl2<br />
[[4ds8]] – AtPP2C + Pyl3 + Mn<br />
[[3rt0]] – AtPP2C (mutant) + Pyl10<br />
[[3qn1]], [[3zvu]] – AtPP2C + Pyr1<br />
[[3ujg]] – AtPP2C + SNRK2<br />
[[3ujk]] – AtPP2C <br />
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