2nxz: Difference between revisions
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[[Image:2nxz.gif|left|200px]]<br /> | [[Image:2nxz.gif|left|200px]]<br /><applet load="2nxz" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2nxz" size=" | |||
caption="2nxz, resolution 2.04Å" /> | caption="2nxz, resolution 2.04Å" /> | ||
'''HIV-1 gp120 Envelope Glycoprotein (T257S, S334A, S375W) Complexed with CD4 and Antibody 17b'''<br /> | '''HIV-1 gp120 Envelope Glycoprotein (T257S, S334A, S375W) Complexed with CD4 and Antibody 17b'''<br /> | ||
==Overview== | ==Overview== | ||
The remarkable diversity, glycosylation and conformational flexibility of | The remarkable diversity, glycosylation and conformational flexibility of the human immunodeficiency virus type 1 (HIV-1) envelope (Env), including substantial rearrangement of the gp120 glycoprotein upon binding the CD4 receptor, allow it to evade antibody-mediated neutralization. Despite this complexity, the HIV-1 Env must retain conserved determinants that mediate CD4 binding. To evaluate how these determinants might provide opportunities for antibody recognition, we created variants of gp120 stabilized in the CD4-bound state, assessed binding of CD4 and of receptor-binding-site antibodies, and determined the structure at 2.3 A resolution of the broadly neutralizing antibody b12 in complex with gp120. b12 binds to a conformationally invariant surface that overlaps a distinct subset of the CD4-binding site. This surface is involved in the metastable attachment of CD4, before the gp120 rearrangement required for stable engagement. A site of vulnerability, related to a functional requirement for efficient association with CD4, can therefore be targeted by antibody to neutralize HIV-1. | ||
==Disease== | |||
Known diseases associated with this structure: CD4 lymphocyte deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=186940 186940]], Lupus erythematosus, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=186940 186940]] | |||
==About this Structure== | ==About this Structure== | ||
2NXZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] with NAG, SUC, EDO, HEZ and IPA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2NXZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=SUC:'>SUC</scene>, <scene name='pdbligand=EDO:'>EDO</scene>, <scene name='pdbligand=HEZ:'>HEZ</scene> and <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NXZ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arthos, J.]] | [[Category: Arthos, J.]] | ||
[[Category: Burton, D | [[Category: Burton, D R.]] | ||
[[Category: Dey, B.]] | [[Category: Dey, B.]] | ||
[[Category: Dimitrov, D | [[Category: Dimitrov, D S.]] | ||
[[Category: Hessell, A | [[Category: Hessell, A J.]] | ||
[[Category: Kwong, P | [[Category: Kwong, P D.]] | ||
[[Category: Nabel, G | [[Category: Nabel, G J.]] | ||
[[Category: Ryk, D | [[Category: Ryk, D Van.]] | ||
[[Category: Sodroski, J.]] | [[Category: Sodroski, J.]] | ||
[[Category: Wyatt, R.]] | [[Category: Wyatt, R.]] | ||
[[Category: Xiang, S | [[Category: Xiang, S H.]] | ||
[[Category: Xu, L.]] | [[Category: Xu, L.]] | ||
[[Category: Yang, X.]] | [[Category: Yang, X.]] | ||
[[Category: Zhang, M | [[Category: Zhang, M Y.]] | ||
[[Category: Zhou, T.]] | [[Category: Zhou, T.]] | ||
[[Category: Zwick, M | [[Category: Zwick, M B.]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
[[Category: HEZ]] | [[Category: HEZ]] | ||
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[[Category: hiv]] | [[Category: hiv]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:12:20 2008'' | ||