Sandbox 125: Difference between revisions

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Wayne Decatur, Student, Marisa L. VanBrakle, Allison Granberry

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 <font size=3> '''Kapβ2 Binding and Conformational Change''' </font>
-The NLSs located on KapB2 cargos are named the PY-NLS and they bind to the C-terminal arch of KapB2.
+The NLSs located on KapB2 cargos are named the PY-NLS (Proline/Tyrosine-Nuclear Localization Signal) and they bind to the C-terminal arch of KapB2.
 Recognition of the PY-NLS by Kapβ2 follows certain guidelines:
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 (ii) PY-NLS has an overall positive charge allowing for electrostatic compatibility with Kapβ2.
-(iii) General sequence for the PY-NLS is a hydrophobic basic motif and a R-X2-5-P-Y motif at the C-terminus.
+(iii) General sequence for the PY-NLS is a hydrophobic basic motif and a RX2-5PY motif at the C-terminus.
 The PY-NLS of 2H4M contains a hydrophobic rather than a basic N-terminal motif. Hydrophobic interactions at the N-terminal motif of the PY-NLS include:
-<scene name='37/372725/Kapb2_hydrophobic_interactions/3'>Phe273, Gly274, Pro275 and Met276 of the NLS with Trp730 and Ile773 of KapB2</scene>. Interactions of the C-terminal R-X2-5-P-Y motif of the NLS include: Arg284 of the NLS with Glu509 and Asp543 of Kapβ2;  Pro288 and Tyr289 of the NLS with Ala380, Ala381, Asp384, Leu419, Ile457, Trp460 and Arg464 of Kapβ2.
+<scene name='37/372725/Kapb2_hydrophobic_interactions/3'>Phe273, Gly274, Pro275 and Met276 of the NLS with Trp730 and Ile773 of KapB2</scene>. Interactions of the C-terminal RX2-5PY motif of the NLS include: Arg284 of the NLS with Glu509 and Asp543 of Kapβ2;  Pro288 and Tyr289 of the NLS with Ala380, Ala381, Asp384, Leu419, Ile457, Trp460 and Arg464 of Kapβ2.
 Upon binding Kapβ2,the NLS gains structure, conforms to and makes contact with the internal surface of the KapB2 C-terminal arch.
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