2nqc: Difference between revisions
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==Overview== | ==Overview== | ||
Filamin C is a dimeric, actin-binding protein involved in organization of | Filamin C is a dimeric, actin-binding protein involved in organization of cortical cytoskeleton and of the sarcomere. We performed crystallographic, small-angle X-ray scattering and analytical ultracentrifugation experiments on the constructs containing carboxy-terminal domains of the protein (domains 23-24 and 19-21). The crystal structure of domain 23 of filamin C showed that the protein adopts the expected immunoglobulin (Ig)-like fold. Small-angle X-ray scattering experiments performed on filamin C tandem Ig-like domains 23 and 24 reveal a dimer that is formed by domain 24 and that domain 23 has little interactions with itself or with domain 24, while the analytical ultracentrifugation experiments showed that the filamin C domains 19-21 form elongated monomers in diluted solutions. | ||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
Crystal structure of human filamin C domain 23 and small angle scattering model for filamin C 23-24 dimer., Sjekloca L, Pudas R, Sjoblom B, Konarev P, Carugo O, Rybin V, Kiema TR, Svergun D, Ylanne J, Carugo | Crystal structure of human filamin C domain 23 and small angle scattering model for filamin C 23-24 dimer., Sjekloca L, Pudas R, Sjoblom B, Konarev P, Carugo O, Rybin V, Kiema TR, Svergun D, Ylanne J, Djinovic Carugo K, J Mol Biol. 2007 May 11;368(4):1011-23. Epub 2007 Feb 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17379241 17379241] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Carugo, O.]] | [[Category: Carugo, O.]] | ||
[[Category: Djinovic-Carugo, K.]] | [[Category: Djinovic-Carugo, K.]] | ||
[[Category: Kiema, T | [[Category: Kiema, T R.]] | ||
[[Category: Konarev, P.]] | [[Category: Konarev, P.]] | ||
[[Category: Pudas, R.]] | [[Category: Pudas, R.]] | ||
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[[Category: metal binding]] | [[Category: metal binding]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:09:47 2008'' | ||
Revision as of 19:10, 21 February 2008
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Crystal structure of ig-like domain 23 from human filamin C
OverviewOverview
Filamin C is a dimeric, actin-binding protein involved in organization of cortical cytoskeleton and of the sarcomere. We performed crystallographic, small-angle X-ray scattering and analytical ultracentrifugation experiments on the constructs containing carboxy-terminal domains of the protein (domains 23-24 and 19-21). The crystal structure of domain 23 of filamin C showed that the protein adopts the expected immunoglobulin (Ig)-like fold. Small-angle X-ray scattering experiments performed on filamin C tandem Ig-like domains 23 and 24 reveal a dimer that is formed by domain 24 and that domain 23 has little interactions with itself or with domain 24, while the analytical ultracentrifugation experiments showed that the filamin C domains 19-21 form elongated monomers in diluted solutions.
About this StructureAbout this Structure
2NQC is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of human filamin C domain 23 and small angle scattering model for filamin C 23-24 dimer., Sjekloca L, Pudas R, Sjoblom B, Konarev P, Carugo O, Rybin V, Kiema TR, Svergun D, Ylanne J, Djinovic Carugo K, J Mol Biol. 2007 May 11;368(4):1011-23. Epub 2007 Feb 20. PMID:17379241
Page seeded by OCA on Thu Feb 21 18:09:47 2008