2msb: Difference between revisions
New page: left|200px<br /><applet load="2msb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2msb, resolution 1.7Å" /> '''STRUCTURE OF A C-TYPE... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2msb.jpg|left|200px]]<br /><applet load="2msb" size=" | [[Image:2msb.jpg|left|200px]]<br /><applet load="2msb" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2msb, resolution 1.7Å" /> | caption="2msb, resolution 1.7Å" /> | ||
'''STRUCTURE OF A C-TYPE MANNOSE-BINDING PROTEIN COMPLEXED WITH AN OLIGOSACCHARIDE'''<br /> | '''STRUCTURE OF A C-TYPE MANNOSE-BINDING PROTEIN COMPLEXED WITH AN OLIGOSACCHARIDE'''<br /> | ||
==Overview== | ==Overview== | ||
C-type (Ca(2+)-dependent) animal lectins such as mannose-binding proteins | C-type (Ca(2+)-dependent) animal lectins such as mannose-binding proteins mediate many cell-surface carbohydrate-recognition events. The crystal structure at 1.7 A resolution of the carbohydrate-recognition domain of rat mannose-binding protein complexed with an oligomannose asparaginyl-oligosaccharide reveals that Ca2+ forms coordination bonds with the carbohydrate ligand. Carbohydrate specificity is determined by a network of coordination and hydrogen bonds that stabilizes the ternary complex of protein, Ca2+ and sugar. Two branches of the oligosaccharide crosslink neighbouring carbohydrate-recognition domains in the crystal, enabling multivalent binding to a single oligosaccharide chain to be visualized directly. | ||
==About this Structure== | ==About this Structure== | ||
2MSB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2MSB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSB OCA]. | ||
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Drickamer, K.]] | [[Category: Drickamer, K.]] | ||
[[Category: Hendrickson, W | [[Category: Hendrickson, W A.]] | ||
[[Category: Weis, W | [[Category: Weis, W I.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: lectin]] | [[Category: lectin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:07:54 2008'' | ||
Revision as of 19:07, 21 February 2008
|
STRUCTURE OF A C-TYPE MANNOSE-BINDING PROTEIN COMPLEXED WITH AN OLIGOSACCHARIDE
OverviewOverview
C-type (Ca(2+)-dependent) animal lectins such as mannose-binding proteins mediate many cell-surface carbohydrate-recognition events. The crystal structure at 1.7 A resolution of the carbohydrate-recognition domain of rat mannose-binding protein complexed with an oligomannose asparaginyl-oligosaccharide reveals that Ca2+ forms coordination bonds with the carbohydrate ligand. Carbohydrate specificity is determined by a network of coordination and hydrogen bonds that stabilizes the ternary complex of protein, Ca2+ and sugar. Two branches of the oligosaccharide crosslink neighbouring carbohydrate-recognition domains in the crystal, enabling multivalent binding to a single oligosaccharide chain to be visualized directly.
About this StructureAbout this Structure
2MSB is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a C-type mannose-binding protein complexed with an oligosaccharide., Weis WI, Drickamer K, Hendrickson WA, Nature. 1992 Nov 12;360(6400):127-34. PMID:1436090
Page seeded by OCA on Thu Feb 21 18:07:54 2008