2mjp: Difference between revisions

Revision as of 19:07, 21 February 2008

STRUCTURE-BASED IDENTIFICATION OF THE BIOCHEMICAL FUNCTION OF A HYPOTHETICAL PROTEIN FROM METHANOCOCCUS JANNASCHII:MJ0226

OverviewOverview

Almost half of the entire set of predicted genomic products from Methanococcus jannaschii are classified as functionally unknown hypothetical proteins. We present a structure-based identification of the biochemical function of a protein with an as yet unknown function from a M. jannaschii gene, Mj0226. The crystal structure of Mj0226 protein determined at 2.2 A resolution reveals that the protein is a homodimer and each monomer folds into an elongated alpha/beta structure of a new fold family. Comparisons of Mj0226 protein with protein structures in the database, however, indicate that one part of the protein is homologous to some of the nucleotide-binding proteins. Biochemical analysis shows that Mj0226 protein is a novel nucleotide triphosphatase that can efficiently hydrolyze nonstandard nucleotides such as XTP to XMP or ITP to IMP, but not the standard nucleotides, in the presence of Mg2+ or Mn2+ ions.

About this StructureAbout this Structure

2MJP is a Single protein structure of sequence from Methanocaldococcus jannaschii with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Structure-based identification of a novel NTPase from Methanococcus jannaschii., Hwang KY, Chung JH, Kim SH, Han YS, Cho Y, Nat Struct Biol. 1999 Jul;6(7):691-6. PMID:10404228

Page seeded by OCA on Thu Feb 21 18:07:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2mjp.gif|left|200px]]<br /><applet load="2mjp" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2mjp.gif|left|200px]]<br /><applet load="2mjp" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2mjp, resolution 2.2&Aring;" />
 '''STRUCTURE-BASED IDENTIFICATION OF THE BIOCHEMICAL FUNCTION OF A HYPOTHETICAL PROTEIN FROM METHANOCOCCUS JANNASCHII:MJ0226'''<br />
 ==Overview==
-Almost half of the entire set of predicted genomic products from, Methanococcus jannaschii are classified as functionally unknown, hypothetical proteins. We present a structure-based identification of the, biochemical function of a protein with an as yet unknown function from a, M. jannaschii gene, Mj0226. The crystal structure of Mj0226 protein, determined at 2.2 A resolution reveals that the protein is a homodimer and, each monomer folds into an elongated alpha/beta structure of a new fold, family. Comparisons of Mj0226 protein with protein structures in the, database, however, indicate that one part of the protein is homologous to, some of the nucleotide-binding proteins. Biochemical analysis shows that, Mj0226 protein is a novel nucleotide triphosphatase that can efficiently, hydrolyze nonstandard nucleotides such as XTP to XMP or ITP to IMP, but, not the standard nucleotides, in the presence of Mg2+ or Mn2+ ions.
+Almost half of the entire set of predicted genomic products from Methanococcus jannaschii are classified as functionally unknown hypothetical proteins. We present a structure-based identification of the biochemical function of a protein with an as yet unknown function from a M. jannaschii gene, Mj0226. The crystal structure of Mj0226 protein determined at 2.2 A resolution reveals that the protein is a homodimer and each monomer folds into an elongated alpha/beta structure of a new fold family. Comparisons of Mj0226 protein with protein structures in the database, however, indicate that one part of the protein is homologous to some of the nucleotide-binding proteins. Biochemical analysis shows that Mj0226 protein is a novel nucleotide triphosphatase that can efficiently hydrolyze nonstandard nucleotides such as XTP to XMP or ITP to IMP, but not the standard nucleotides, in the presence of Mg2+ or Mn2+ ions.
 ==About this Structure==
-MJP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with ANP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2MJP OCA].
+MJP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJP OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Methanocaldococcus jannaschii]]
 [[Category: Single protein]]
-[[Category: BSGC, Berkeley.Structural.Genomics.Center.]]
+[[Category: BSGC, Berkeley Structural Genomics Center.]]
 [[Category: Cho, Y.]]
-[[Category: Chung, J.H.]]
+[[Category: Chung, J H.]]
-[[Category: Han, Y.S.]]
+[[Category: Han, Y S.]]
-[[Category: Hwang, K.Y.]]
+[[Category: Hwang, K Y.]]
-[[Category: Kim, S.H.]]
+[[Category: Kim, S H.]]
 [[Category: ANP]]
 [[Category: berkeley structural genomics center]]
@@ Line 28: / Line 28: @@
 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:45:10 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:07:44 2008''