2jaa: Difference between revisions
New page: left|200px<br /><applet load="2jaa" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jaa, resolution 3.1Å" /> '''SEMET SUBSTITUTED SHI... |
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[[Image:2jaa.jpg|left|200px]]<br /><applet load="2jaa" size=" | [[Image:2jaa.jpg|left|200px]]<br /><applet load="2jaa" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2jaa, resolution 3.1Å" /> | caption="2jaa, resolution 3.1Å" /> | ||
'''SEMET SUBSTITUTED SHIGELLA FLEXNERI IPAD'''<br /> | '''SEMET SUBSTITUTED SHIGELLA FLEXNERI IPAD'''<br /> | ||
==Overview== | ==Overview== | ||
Bacteria expressing type III secretion systems (T3SS) have been | Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. The T3SS is composed of a basal body, which traverses both bacterial membranes, and an external needle through which effector proteins are secreted. We report multiple crystal structures of two proteins that sit at the tip of the needle and are essential for virulence: IpaD from Shigella flexneri and BipD from Burkholderia pseudomallei. The structures reveal that the N-terminal domains of the molecules are intramolecular chaperones that prevent premature oligomerization, as well as sharing structural homology with proteins involved in eukaryotic actin rearrangement. Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure. | ||
==About this Structure== | ==About this Structure== | ||
2JAA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http:// | 2JAA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JAA OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Birket, S.]] | [[Category: Birket, S.]] | ||
[[Category: Blocker, A.]] | [[Category: Blocker, A.]] | ||
[[Category: Deane, J | [[Category: Deane, J E.]] | ||
[[Category: Espina, M.]] | [[Category: Espina, M.]] | ||
[[Category: Field, T.]] | [[Category: Field, T.]] | ||
[[Category: Galyov, E | [[Category: Galyov, E E.]] | ||
[[Category: Johnson, S.]] | [[Category: Johnson, S.]] | ||
[[Category: Lea, S | [[Category: Lea, S M.]] | ||
[[Category: Olive, A.]] | [[Category: Olive, A.]] | ||
[[Category: Picking, W | [[Category: Picking, W D.]] | ||
[[Category: Picking, W | [[Category: Picking, W L.]] | ||
[[Category: Roversi, P.]] | [[Category: Roversi, P.]] | ||
[[Category: cell invasion]] | [[Category: cell invasion]] | ||
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[[Category: virulence]] | [[Category: virulence]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:00:59 2008'' | ||
Revision as of 19:01, 21 February 2008
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SEMET SUBSTITUTED SHIGELLA FLEXNERI IPAD
OverviewOverview
Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. The T3SS is composed of a basal body, which traverses both bacterial membranes, and an external needle through which effector proteins are secreted. We report multiple crystal structures of two proteins that sit at the tip of the needle and are essential for virulence: IpaD from Shigella flexneri and BipD from Burkholderia pseudomallei. The structures reveal that the N-terminal domains of the molecules are intramolecular chaperones that prevent premature oligomerization, as well as sharing structural homology with proteins involved in eukaryotic actin rearrangement. Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure.
About this StructureAbout this Structure
2JAA is a Single protein structure of sequence from Shigella flexneri. Full crystallographic information is available from OCA.
ReferenceReference
Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD., Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM, J Biol Chem. 2007 Feb 9;282(6):4035-44. Epub 2006 Oct 31. PMID:17077085
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