Ubiquitin conjugating enzyme

<StructureSection load='1z2u' size='350' side='right' caption='Structure of ubiquitin conjugating enzyme 2 comlex with butanediol and Cl-, Na+ ions (PDB entry 1z2u)' scene=>

Ubiquitin conjugating enzyme (Ubc) or E2 enzyme catalyzes the second step in the ubiquitination of a protein tagged to be degraded by the proteasome. Ubiquitin activating enzyme (E1) and ATP produce a C-terminal acyl adenylated ubiquitin molecule which binds to ubiquitin conjugating enzyme (Ubc) cysteine. The Ubc then binds to ubiquitin ligase (E3) via a conserved binding region. E3 catalyzes the transfer of ubiquitin from the Ubc-ubiquitin complex to a lysine residue of the target protein. Ubc13 makes a catalytically active heterodimer with MMS2.

3D Structures of ubiquitin conjugating enzyme3D Structures of ubiquitin conjugating enzyme

Updated on 07-August-2013

2aak – AtUbc – Arabidopsis thaliana
2e2c – Ubc – Spisula solidissima
1q34 – CeUbc – Caenorhabidis elegans
2qcq, 1fzy – yUbc – yeast
1jas – hUbc – human
2fo3 – Ubc – Plasmodium vivax
2h2y – PfUbc – Plasmodium falciparum
1fxt – yUbc + ubiquitin

Ubc1

1tte – yUbc (mutant)
2aak – AtUbc
1z3d – CeUbc

Ubc2

1ayz – yUbc
1z2u – CeUbc

Ubc4

1qcq – yUbc
4ii2 – Ubc (mutant) + E1 + ATP – fission yeast

Ubc5

2esk, 2c4o, 2clw, 3l1y, 3tgd, 2c4p, 1x23 – hUbc
2eso, 2esp, 2esq – hUbc (mutant)
1w4u – hUbc - NMR

Ubc5 complexes

1ur6 – hUbc + Cnot4 - NMR
3eb6 – Ubc + baculoviral IAP repeat-containing protein- frog
4auq – hUbc (mutant) + baculoviral IAP repeat-containing protein + ubiquitin
3ptf, 4ddg, 4ddi – hUbc + ubiquitin
2fuh – hUbc + ubiquitin - NMR
3a33, 3ugb – hUbc (mutant) + ubiquitin
3jvz, 3jw0 – hUbc (mutant) + E3 NEDD4-2 + ubiquitin
4ap4 – hUbc + E3 Rnf4 + ubiquitin
3oj4 – hUbc + tumor necrosis factor α-induced protein 3 + ubiquitin
4a49 – hUbc + E3 Cbl
4a4b, 4a4c – hUbc + E3 Cbl + ZAP-70 peptide
3l1z – hUbc + ubiquitin conjugating factor
3rpg – hUbc + E3 Ring2 + polycomb complex protein Bmi-1
3zni – hUbc + ubiquitin + + E3 Cbl + ZAP-70 peptide

Ubc6

4jjq – hUbc + ubiquitin carboxyl terminal hydrolase

Ubc7

2ucz – yUbc
2awf, 2cyx – hUbc
1c4z – hUbc + E3 HECT domain
1fbv – hUbc + E3 Cbl + ZAP-70 peptide
4jqu – hUbc + U7BR
3sqv – hUbc + secreted effector protein
3sy2 – hUbc + E3 Sopa

Ubc8

1y6l, 1wzw – hUbc
2kjh – hUbc + ubiuqitin

Ubc9

See SUMO conjugating enzyme Ubc9

Ubc10

1i7k – hUbc (mutant)

Ubc12 (NEDD8 conjugating enzyme)

3o2u – yUbc
1tt5 – hUbc + E1 Uba3, APPBP1 subunits
1y8x – hUbc + E1 NEDD8
2nvu – hUbc + E1 NEDD8 + NEDD8
3tdu – hUbc + cullin-1 + Dcn1-like protein
4gao – hUbc + Dcn1-like protein
3tdi – hUbc + defective in cullin neddylation protein

Ubc13

1jbb – yUbc
1jat – yUbc/Mms2
1j74 – hMms2
1j7d – hUbc/Mms2
4fh1 – hUbc (mutant)

2c2v – hUbc + CHIP + Uev1a
3hct, 3hcu – hUbc + TNF receptor associated factor 6
4dhi – CeUbc + OTUB1
4dhi, 4dhz – hUbc + OTUB1 + ubiquitin + ubiquitin aldehyde
4epo – hUbc/Mms2 + E3 Rnf8
3von – hUbc/Mms2 + OTUB1
1zgu – hMms2 + ubiquitin - NMR
2gmi – hUbc/Mms2 (mutant) + ubiquitin
4ip3, 3w31 – hUbc + Orf169b
2oxq – Ubc + CHIP U-box - zebrafish

Ubc B

2y4w – hUbc - NMR

Ubc D1

2hyo – hUbc + E3 Mylip

Ubc E1

3bzh – hUbc

Ubc G2

3fsh – hUbc + autocrine motility factor receptor
2kly – hUbc - NMR

Ubc H

2z5d – hUbc

Ubc J2

2f4w – hUbc

Ubc Q

2qgx – hUbc

Ubc R1

3rz3 – hUbc + inhibitor

Ubc S

1zdn – hUbc

Ubc Uev-1

2a4d – hUbc catalytic domain
2hlw – hUbc catalytic domain - NMR
3e95 – PfUbc + ubiquitin carrier protein

Ubc E2-25KDa (Huntington interacting protein 2 HIP-2)

1yla, 2bep – hHIP-2
3e46 – hHIP-2 (mutant)
2bf8 – hHIP-2 + SUMO
2o25 – hHIP-2 + Ubc9
3f92 – hHIP-2/azorectase (mutant)
3k9o, 3k9p – hHIP-2 + ubiquitin