2j6v: Difference between revisions
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==Overview== | ==Overview== | ||
The ultraviolet damage endonuclease (UVDE) performs the initial step in an | The ultraviolet damage endonuclease (UVDE) performs the initial step in an alternative excision repair pathway of UV-induced DNA damage, nicking immediately adjacent to the 5' phosphate of the damaged nucleotides. Unique for a single-protein DNA repair endonuclease, it can detect different types of damage. Here we show that Thermus thermophilus UVDE shares some essential structural features with Endo IV, an enzyme from the base excision repair pathway that exclusively nicks at abasic sites. A comparison between the structures indicates how DNA is bound by UVDE, how UVDE may recognize damage, and which of its residues are involved in catalysis. Furthermore, the comparison suggests an elegant explanation of UVDE's potential to recognize different types of damage. Incision assays including point mutants of UVDE confirmed the relevance of these conclusions. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Abrahams, J | [[Category: Abrahams, J P.]] | ||
[[Category: Goossen, N.]] | [[Category: Goossen, N.]] | ||
[[Category: Pannu, N | [[Category: Pannu, N S.]] | ||
[[Category: Paspaleva, K.]] | [[Category: Paspaleva, K.]] | ||
[[Category: Thomassen, E | [[Category: Thomassen, E A.J.]] | ||
[[Category: MN]] | [[Category: MN]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
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[[Category: uvde]] | [[Category: uvde]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:59:53 2008'' | ||
Revision as of 18:59, 21 February 2008
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CRYSTAL STRUCTURE OF THE DNA REPAIR ENZYME UV DAMAGE ENDONUC
OverviewOverview
The ultraviolet damage endonuclease (UVDE) performs the initial step in an alternative excision repair pathway of UV-induced DNA damage, nicking immediately adjacent to the 5' phosphate of the damaged nucleotides. Unique for a single-protein DNA repair endonuclease, it can detect different types of damage. Here we show that Thermus thermophilus UVDE shares some essential structural features with Endo IV, an enzyme from the base excision repair pathway that exclusively nicks at abasic sites. A comparison between the structures indicates how DNA is bound by UVDE, how UVDE may recognize damage, and which of its residues are involved in catalysis. Furthermore, the comparison suggests an elegant explanation of UVDE's potential to recognize different types of damage. Incision assays including point mutants of UVDE confirmed the relevance of these conclusions.
About this StructureAbout this Structure
2J6V is a Single protein structure of sequence from Thermus thermophilus with and as ligands. Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the DNA repair enzyme ultraviolet damage endonuclease., Paspaleva K, Thomassen E, Pannu NS, Iwai S, Moolenaar GF, Goosen N, Abrahams JP, Structure. 2007 Oct;15(10):1316-24. PMID:17937920
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