2j5h: Difference between revisions
New page: left|200px<br /><applet load="2j5h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j5h" /> '''NMR ANALYSIS OF MOUSE CRIPTO CFC DOMAIN'''<b... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2j5h.jpg|left|200px]]<br /><applet load="2j5h" size=" | [[Image:2j5h.jpg|left|200px]]<br /><applet load="2j5h" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2j5h" /> | caption="2j5h" /> | ||
'''NMR ANALYSIS OF MOUSE CRIPTO CFC DOMAIN'''<br /> | '''NMR ANALYSIS OF MOUSE CRIPTO CFC DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
We report here for the first time the solution structures at pH 3 and pH 6 | We report here for the first time the solution structures at pH 3 and pH 6 of the synthetic CFC domain of mouse Cripto and of the point mutated variant W107A that is unable to bind to the Alk4 Cripto receptor. NMR data confirm that the CFC domain has a C1-C4, C2-C6, C3-C5 disulfide pattern and show that structures are rather flexible and globally extended, with three noncanonical antiparallel strands. His104 and Trp107 side chains protrude from a protein edge and are strongly exposed to solvent, supporting previous evidence of direct involvement in receptor binding. On the opposite molecule side, several nonpolar residues are gathered, forming a large hydrophobic patch that supposedly acts as interface with the cell membrane or the adjacent EGF-like domain. A second hydrophilic patch surrounding His104 and Trp107 is present only in the wild type variant, suggesting a possible involvement in modulating Alk4 recognition. | ||
==About this Structure== | ==About this Structure== | ||
2J5H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ACE and NH2 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2J5H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5H OCA]. | ||
==Reference== | ==Reference== | ||
Solution structure of mouse Cripto CFC domain and its inactive variant Trp107Ala., Calvanese L, Saporito A, Marasco D, D'Auria G, Minchiotti G, Pedone C, Paolillo L, Falcigno L, Ruvo M, J Med Chem. 2006 Nov 30;49(24):7054-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17125258 17125258] | Solution structure of mouse Cripto CFC domain and its inactive variant Trp107Ala., Calvanese L, Saporito A, Marasco D, D'Auria G, Minchiotti G, Pedone C, Paolillo L, Falcigno L, Ruvo M, J Med Chem. 2006 Nov 30;49(24):7054-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17125258 17125258] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Auria, G | [[Category: Auria, G D.]] | ||
[[Category: Calvanese, L.]] | [[Category: Calvanese, L.]] | ||
[[Category: Falcigno, L.]] | [[Category: Falcigno, L.]] | ||
| Line 30: | Line 30: | ||
[[Category: tumour progression]] | [[Category: tumour progression]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:59:26 2008'' | ||
