2j57: Difference between revisions
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==Overview== | ==Overview== | ||
X-ray exposure during crystallographic data collection can result in | X-ray exposure during crystallographic data collection can result in unintended redox changes in proteins containing functionally important redox centers. In order to directly monitor X-ray-derived redox changes in trapped oxidative half-reaction intermediates of Paracoccus denitrificans methylamine dehydrogenase, a commercially available single-crystal UV/Vis microspectrophotometer was installed on-line at the BioCARS beamline 14-BM-C at the Advanced Photon Source, Argonne, USA. Monitoring the redox state of the intermediates during X-ray exposure permitted the creation of a general multi-crystal data collection strategy to generate true structures of each redox intermediate. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Davidson, V.]] | [[Category: Davidson, V.]] | ||
[[Category: Pahl, R.]] | [[Category: Pahl, R.]] | ||
[[Category: Pearson, A | [[Category: Pearson, A R.]] | ||
[[Category: Wilmot, C | [[Category: Wilmot, C M.]] | ||
[[Category: CU]] | [[Category: CU]] | ||
[[Category: copper]] | [[Category: copper]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:59:18 2008'' | ||
Revision as of 18:59, 21 February 2008
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X-RAY REDUCED PARACCOCUS DENITRIFICANS METHYLAMINE DEHYDROGENASE N-QUINOL IN COMPLEX WITH AMICYANIN.
OverviewOverview
X-ray exposure during crystallographic data collection can result in unintended redox changes in proteins containing functionally important redox centers. In order to directly monitor X-ray-derived redox changes in trapped oxidative half-reaction intermediates of Paracoccus denitrificans methylamine dehydrogenase, a commercially available single-crystal UV/Vis microspectrophotometer was installed on-line at the BioCARS beamline 14-BM-C at the Advanced Photon Source, Argonne, USA. Monitoring the redox state of the intermediates during X-ray exposure permitted the creation of a general multi-crystal data collection strategy to generate true structures of each redox intermediate.
About this StructureAbout this Structure
2J57 is a Protein complex structure of sequences from Paracoccus denitrificans with as ligand. Active as Amine dehydrogenase, with EC number 1.4.99.3 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Tracking X-ray-derived redox changes in crystals of a methylamine dehydrogenase/amicyanin complex using single-crystal UV/Vis microspectrophotometry., Pearson AR, Pahl R, Kovaleva EG, Davidson VL, Wilmot CM, J Synchrotron Radiat. 2007 Jan;14(Pt 1):92-8. Epub 2006 Dec 15. PMID:17211075
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