2j0a: Difference between revisions
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==Overview== | ==Overview== | ||
Fringe proteins are beta1,3-N-acetylglucosaminyltransferases that modify | Fringe proteins are beta1,3-N-acetylglucosaminyltransferases that modify Notch receptors, altering their ligand-binding specificity to regulate Notch signaling in development. We present the crystal structure of mouse Manic Fringe bound to UDP and manganese. The structure reveals amino acid residues involved in recognition of donor substrates and catalysis, and a putative binding pocket for acceptor substrates. Mutations of several invariant residues in this pocket impair Fringe activity in vivo. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase]] | [[Category: O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chen, Y | [[Category: Chen, Y W.]] | ||
[[Category: Clausen, H.]] | [[Category: Clausen, H.]] | ||
[[Category: Cohen, S | [[Category: Cohen, S M.]] | ||
[[Category: Conti, E.]] | [[Category: Conti, E.]] | ||
[[Category: Jinek, M.]] | [[Category: Jinek, M.]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:57:56 2008'' | ||
Revision as of 18:57, 21 February 2008
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STRUCTURE OF THE CATALYTIC DOMAIN OF MOUSE MANIC FRINGE
OverviewOverview
Fringe proteins are beta1,3-N-acetylglucosaminyltransferases that modify Notch receptors, altering their ligand-binding specificity to regulate Notch signaling in development. We present the crystal structure of mouse Manic Fringe bound to UDP and manganese. The structure reveals amino acid residues involved in recognition of donor substrates and catalysis, and a putative binding pocket for acceptor substrates. Mutations of several invariant residues in this pocket impair Fringe activity in vivo.
About this StructureAbout this Structure
2J0A is a Single protein structure of sequence from Mus musculus with and as ligands. Active as O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, with EC number 2.4.1.222 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structural insights into the Notch-modifying glycosyltransferase Fringe., Jinek M, Chen YW, Clausen H, Cohen SM, Conti E, Nat Struct Mol Biol. 2006 Oct;13(10):945-6. Epub 2006 Sep 10. PMID:16964258
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