2iz9: Difference between revisions

Revision as of 18:57, 21 February 2008

MS2-RNA HAIRPIN (4ONE-5) COMPLEX

OverviewOverview

The MS2 coat protein binds specifically to an RNA hairpin formed within the viral genome. By soaking different RNA fragments into crystals of MS2 coat protein capsids it is possible to determine the X-ray structure of the RNA-protein complexes formed. Here we present the structure to 2.85 A resolution of a complex between a chemically modified RNA hairpin variant and the MS2 coat protein. This RNA variant has a substitution at the -5 base position, which has been shown previously to be pyrimidine-specific and is a uracil in the wild-type RNA. The modified RNA hairpin contains a pyridin-4-one base (4one) at this position that lacks the exocyclic 2-oxygen eliminating the possibility of forming a hydrogen bond to asparagine A87 in the protein. The 4one complex structure shows an unprecedented major conformational change in the loop region of the RNA, whereas there is almost no change in the conformation of the protein.

About this StructureAbout this Structure

2IZ9 is a Single protein structure of sequence from Enterobacterio phage ms2. This structure supersedes the now removed PDB entry 1DZS. Full crystallographic information is available from OCA.

ReferenceReference

Deletion of a single hydrogen bonding atom from the MS2 RNA operator leads to dramatic rearrangements at the RNA-coat protein interface., Grahn E, Stonehouse NJ, Adams CJ, Fridborg K, Beigelman L, Matulic-Adamic J, Warriner SL, Stockley PG, Liljas L, Nucleic Acids Res. 2000 Dec 1;28(23):4611-6. PMID:11095669

Page seeded by OCA on Thu Feb 21 17:57:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2iz9.gif|left|200px]]<br /><applet load="2iz9" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2iz9.gif|left|200px]]<br /><applet load="2iz9" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2iz9, resolution 2.85&Aring;" />
 '''MS2-RNA HAIRPIN (4ONE-5) COMPLEX'''<br />
 ==Overview==
-The MS2 coat protein binds specifically to an RNA hairpin formed within, the viral genome. By soaking different RNA fragments into crystals of MS2, coat protein capsids it is possible to determine the X-ray structure of, the RNA-protein complexes formed. Here we present the structure to 2.85 A, resolution of a complex between a chemically modified RNA hairpin variant, and the MS2 coat protein. This RNA variant has a substitution at the -5, base position, which has been shown previously to be pyrimidine-specific, and is a uracil in the wild-type RNA. The modified RNA hairpin contains a, pyridin-4-one base (4one) at this position that lacks the exocyclic, 2-oxygen eliminating the possibility of forming a hydrogen bond to, asparagine A87 in the protein. The 4one complex structure shows an, unprecedented major conformational change in the loop region of the RNA, whereas there is almost no change in the conformation of the protein.
+The MS2 coat protein binds specifically to an RNA hairpin formed within the viral genome. By soaking different RNA fragments into crystals of MS2 coat protein capsids it is possible to determine the X-ray structure of the RNA-protein complexes formed. Here we present the structure to 2.85 A resolution of a complex between a chemically modified RNA hairpin variant and the MS2 coat protein. This RNA variant has a substitution at the -5 base position, which has been shown previously to be pyrimidine-specific and is a uracil in the wild-type RNA. The modified RNA hairpin contains a pyridin-4-one base (4one) at this position that lacks the exocyclic 2-oxygen eliminating the possibility of forming a hydrogen bond to asparagine A87 in the protein. The 4one complex structure shows an unprecedented major conformational change in the loop region of the RNA, whereas there is almost no change in the conformation of the protein.
 ==About this Structure==
-IZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. This structure superseeds the now removed PDB entry 1DZS. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IZ9 OCA].
+IZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. This structure supersedes the now removed PDB entry 1DZS. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZ9 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Enterobacterio phage ms2]]
 [[Category: Single protein]]
-[[Category: Adams, C.J.]]
+[[Category: Adams, C J.]]
 [[Category: Beigelman, L.]]
 [[Category: Fridborg, K.]]
@@ Line 19: / Line 19: @@
 [[Category: Liljas, L.]]
 [[Category: Matulic-Adamic, J.]]
-[[Category: Stockley, P.G.]]
+[[Category: Stockley, P G.]]
-[[Category: Stonehouse, N.J.]]
+[[Category: Stonehouse, N J.]]
-[[Category: Warriner, S.L.]]
+[[Category: Warriner, S L.]]
 [[Category: capsid]]
 [[Category: complex (capsid protein/rna hairpin)]]
@@ Line 28: / Line 28: @@
 [[Category: virus]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:28:20 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:57:37 2008''