2iwr: Difference between revisions

Revision as of 18:56, 21 February 2008

GTPASE LIKE DOMAIN OF CENTAURIN GAMMA 1 (HUMAN)

OverviewOverview

Centaurins are a family of proteins that contain GTPase-activating protein domains, with the gamma family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.

About this StructureAbout this Structure

2IWR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The centaurin gamma-1 GTPase-like domain functions as an NTPase., Soundararajan M, Yang X, Elkins JM, Sobott F, Doyle DA, Biochem J. 2007 Feb 1;401(3):679-88. PMID:17037982

Page seeded by OCA on Thu Feb 21 17:56:47 2008

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OCA

@@ Line 4: / Line 4: @@
 ==Overview==
-Centaurins are a family of proteins that contain GTPase-activating protein, domains, with the gamma family members containing in addition a, GTPase-like domain. Centaurins reside mainly in the nucleus and are known, to activate phosphoinositide 3-kinase, a key regulator of cell, proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding, studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like, domain has broader trinucleotide specificity. Alterations within the G4, motif of CENTG1 from the highly conserved NKXD found in typical GTPases to, TQDR result in the loss of specificity, a lower affinity for the, nucleotides and higher turnover rates. These results indicate that the, centaurins could be more accurately classified as NTPases and point to, alternative mechanisms of cell signalling control.
+Centaurins are a family of proteins that contain GTPase-activating protein domains, with the gamma family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Arrowsmith, C.]]
-[[Category: Doyle, D.A.]]
+[[Category: Doyle, D A.]]
 [[Category: Edwards, A.]]
-[[Category: Elkins, J.M.]]
+[[Category: Elkins, J M.]]
 [[Category: Papagrigoriou, E.]]
-[[Category: Sgc, Structural.Genomics.Consortium.]]
+[[Category: Sgc, Structural Genomics Consortium.]]
 [[Category: Soundararajan, M.]]
 [[Category: Sundstrom, M.]]
@@ Line 45: / Line 45: @@
 [[Category: zinc-finger]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:19:26 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:56:47 2008''