4l8r: Difference between revisions
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' | {{STRUCTURE_4l8r| PDB=4l8r | SCENE= }} | ||
===Structure of mrna stem-loop, human stem-loop binding protein and 3'hexo ternary complex=== | |||
{{ABSTRACT_PUBMED_23329046}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/SLBP_HUMAN SLBP_HUMAN]] RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs.<ref>PMID:12588979</ref> <ref>PMID:19155325</ref> [[http://www.uniprot.org/uniprot/ERI1_HUMAN ERI1_HUMAN]] RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.<ref>PMID:14536070</ref> <ref>PMID:16912046</ref> | |||
==About this Structure== | |||
[[4l8r]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4hxh 4hxh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8R OCA]. | |||
==Reference== | |||
<ref group="xtra">PMID:023329046</ref><references group="xtra"/><references/> | |||
[[Category: Homo sapiens]] | |||
[[Category: Tan, D.]] | |||
[[Category: Tong, L.]] | |||
[[Category: 5 8s rrna 3 -end maturation]] | |||
[[Category: Histone mrna 3'-end processing]] | |||
[[Category: Histone mrna translation]] | |||
[[Category: Lsm11]] | |||
[[Category: Microrna homeostasis]] | |||
[[Category: Nucleus]] | |||
[[Category: Rna-rna binding protein-hydrolase complex]] | |||
[[Category: Zfp100]] | |||
Revision as of 18:35, 10 July 2013
Structure of mrna stem-loop, human stem-loop binding protein and 3'hexo ternary complexStructure of mrna stem-loop, human stem-loop binding protein and 3'hexo ternary complex
Template:ABSTRACT PUBMED 23329046
FunctionFunction
[SLBP_HUMAN] RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs.[1] [2] [ERI1_HUMAN] RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.[3] [4]
About this StructureAbout this Structure
4l8r is a 5 chain structure with sequence from Homo sapiens. This structure supersedes the now removed PDB entry 4hxh. Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Tan D, Marzluff WF, Dominski Z, Tong L. Structure of histone mRNA stem-loop, human stem-loop binding protein, and 3'hExo ternary complex. Science. 2013 Jan 18;339(6117):318-21. doi: 10.1126/science.1228705. PMID:23329046 doi:10.1126/science.1228705
- ↑ Zheng L, Dominski Z, Yang XC, Elms P, Raska CS, Borchers CH, Marzluff WF. Phosphorylation of stem-loop binding protein (SLBP) on two threonines triggers degradation of SLBP, the sole cell cycle-regulated factor required for regulation of histone mRNA processing, at the end of S phase. Mol Cell Biol. 2003 Mar;23(5):1590-601. PMID:12588979
- ↑ Sullivan KD, Mullen TE, Marzluff WF, Wagner EJ. Knockdown of SLBP results in nuclear retention of histone mRNA. RNA. 2009 Mar;15(3):459-72. doi: 10.1261/rna.1205409. Epub 2009 Jan 20. PMID:19155325 doi:10.1261/rna.1205409
- ↑ Dominski Z, Yang XC, Kaygun H, Dadlez M, Marzluff WF. A 3' exonuclease that specifically interacts with the 3' end of histone mRNA. Mol Cell. 2003 Aug;12(2):295-305. PMID:14536070
- ↑ Yang XC, Purdy M, Marzluff WF, Dominski Z. Characterization of 3'hExo, a 3' exonuclease specifically interacting with the 3' end of histone mRNA. J Biol Chem. 2006 Oct 13;281(41):30447-54. Epub 2006 Aug 15. PMID:16912046 doi:10.1074/jbc.M602947200