Ferredoxin: Difference between revisions
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[[Image: | <StructureSection load='1vjw' size='450' side='right' scene='Journal:JBIC:10/Cv1/2' caption=''> | ||
[[Image:2z8q.png|left|150px]]<br /> | |||
[[Ferredoxin]] (Fd) is found in chloroplasts which mediates electron transfer and contains an iron-sulfur cluster. It is involved in the photosynthesis process where its iron atoms accept or discharge electrons when they are being oxidized or reduced. The iron-sulfur cluster can contain 2Fe-2S and is termed plant-like or 3Fe-4S or 4Fe-4S clusters | [[Ferredoxin]] (Fd) is found in chloroplasts which mediates electron transfer and contains an iron-sulfur cluster. It is involved in the photosynthesis process where its iron atoms accept or discharge electrons when they are being oxidized or reduced. The iron-sulfur cluster can contain 2Fe-2S and is termed plant-like or 3Fe-4S or 4Fe-4S clusters. '''Adrenodoxin''' (ADR) is a ferredoxin containing a 2Fe-2S group involved in electron transfer from NADPH+ to a cytochrome P-450 in the adrenal gland. '''Putidaredoxin''' (PUT) and '''terpredoxin''' (TER) are involved in the same reaction in bacteria and contain a 2Fe-2S group. | ||
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==D14C variant of ''Pyrococcus furiosus'' ferredoxin<ref>DOI 10.1007/s00775-011-0778-7</ref>== | ==D14C variant of ''Pyrococcus furiosus'' ferredoxin<ref>DOI 10.1007/s00775-011-0778-7</ref>== | ||
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Two forms of D14C [3Fe-4S] ''Pyrococcus furiosus'' ferredoxin are obtained when purified at pH 8.0: a monomer and a dimer connected by an intermolecular disulfide bond (see static image at the left). When purified at pH 5.8, only the monomer is obtained. The [3Fe-4S] form diffracted to 2.8 Å resolution and showed only the <scene name='Journal:JBIC:10/Cv1/13'>monomeric form, which resembles molecule A of D14C [4Fe-4S] Pyrococcus furiosus ferredoxin</scene>. Crystal packing in <scene name='Journal:JBIC:10/Cv2/7'>D14C [3Fe-4S] ferredoxin is as extended beta-sheet dimers of adjacent molecules</scene> (shown in <font color='red'><b>red</b></font> and <font color='orange'><b>orange</b></font>), which is the same as <scene name='Journal:JBIC:10/Cv2/9'>WT [3Fe-4S] P. furiosus ferredoxin</scene> ([[1sj1]], shown in <font color='blue'><b>blue</b></font> and <font color='cyan'><b>cyan</b></font>) even though the space groups are different (see also corresponding side views for <scene name='Journal:JBIC:10/Cv2/8'>D14C [3Fe-4S]</scene>) and <scene name='Journal:JBIC:10/Cv2/10'>WT [3Fe-4S]</scene>). | Two forms of D14C [3Fe-4S] ''Pyrococcus furiosus'' ferredoxin are obtained when purified at pH 8.0: a monomer and a dimer connected by an intermolecular disulfide bond (see static image at the left). When purified at pH 5.8, only the monomer is obtained. The [3Fe-4S] form diffracted to 2.8 Å resolution and showed only the <scene name='Journal:JBIC:10/Cv1/13'>monomeric form, which resembles molecule A of D14C [4Fe-4S] Pyrococcus furiosus ferredoxin</scene>. Crystal packing in <scene name='Journal:JBIC:10/Cv2/7'>D14C [3Fe-4S] ferredoxin is as extended beta-sheet dimers of adjacent molecules</scene> (shown in <font color='red'><b>red</b></font> and <font color='orange'><b>orange</b></font>), which is the same as <scene name='Journal:JBIC:10/Cv2/9'>WT [3Fe-4S] P. furiosus ferredoxin</scene> ([[1sj1]], shown in <font color='blue'><b>blue</b></font> and <font color='cyan'><b>cyan</b></font>) even though the space groups are different (see also corresponding side views for <scene name='Journal:JBIC:10/Cv2/8'>D14C [3Fe-4S]</scene>) and <scene name='Journal:JBIC:10/Cv2/10'>WT [3Fe-4S]</scene>). | ||
==ISC-like [2Fe-2S] ferredoxin (FdxB) dimer from ''Pseudomonas putida'' JCM 20004<ref>DOI:10.1007/s00775-011-0793-8</ref>== | ==ISC-like [2Fe-2S] ferredoxin (FdxB) dimer from ''Pseudomonas putida'' JCM 20004<ref>DOI:10.1007/s00775-011-0793-8</ref>== | ||
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</StructureSection> | </StructureSection> | ||
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===3D structures of ferredoxin=== | ===3D structures of ferredoxin=== | ||