2i91: Difference between revisions

Revision as of 18:50, 21 February 2008

60kDa Ro autoantigen in complex with a fragment of misfolded RNA

OverviewOverview

The Ro autoantigen is ring-shaped, binds misfolded noncoding RNAs and is proposed to function in quality control. Here we determine how Ro interacts with misfolded RNAs. Binding of Ro to misfolded precursor (pre)-5S ribosomal RNA requires a single-stranded 3' end and helical elements. As mutating most sequences of the helices and tail results in modest decreases in binding, Ro may be able to associate with a range of RNAs. Ro binds several other RNAs that contain single-stranded tails. A crystal structure of Ro bound to a misfolded pre-5S rRNA fragment reveals that the tail inserts into the cavity, while a helix binds on the surface. Most contacts of Ro with the helix are to the backbone. Mutagenesis reveals that Ro has an extensive RNA-binding surface. We propose that Ro uses this surface to scavenge RNAs that fail to bind their specific RNA-binding proteins.

About this StructureAbout this Structure

2I91 is a Single protein structure of sequence from Xenopus laevis with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Structural and biochemical basis for misfolded RNA recognition by the Ro autoantigen., Fuchs G, Stein AJ, Fu C, Reinisch KM, Wolin SL, Nat Struct Mol Biol. 2006 Nov;13(11):1002-9. Epub 2006 Oct 15. PMID:17041599

Page seeded by OCA on Thu Feb 21 17:50:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2i91.gif|left|200px]]<br /><applet load="2i91" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2i91.gif|left|200px]]<br /><applet load="2i91" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2i91, resolution 2.65&Aring;" />
 '''60kDa Ro autoantigen in complex with a fragment of misfolded RNA'''<br />
 ==Overview==
-The Ro autoantigen is ring-shaped, binds misfolded noncoding RNAs and is, proposed to function in quality control. Here we determine how Ro, interacts with misfolded RNAs. Binding of Ro to misfolded precursor, (pre)-5S ribosomal RNA requires a single-stranded 3' end and helical, elements. As mutating most sequences of the helices and tail results in, modest decreases in binding, Ro may be able to associate with a range of, RNAs. Ro binds several other RNAs that contain single-stranded tails. A, crystal structure of Ro bound to a misfolded pre-5S rRNA fragment reveals, that the tail inserts into the cavity, while a helix binds on the surface., Most contacts of Ro with the helix are to the backbone. Mutagenesis, reveals that Ro has an extensive RNA-binding surface. We propose that Ro, uses this surface to scavenge RNAs that fail to bind their specific, RNA-binding proteins.
+The Ro autoantigen is ring-shaped, binds misfolded noncoding RNAs and is proposed to function in quality control. Here we determine how Ro interacts with misfolded RNAs. Binding of Ro to misfolded precursor (pre)-5S ribosomal RNA requires a single-stranded 3' end and helical elements. As mutating most sequences of the helices and tail results in modest decreases in binding, Ro may be able to associate with a range of RNAs. Ro binds several other RNAs that contain single-stranded tails. A crystal structure of Ro bound to a misfolded pre-5S rRNA fragment reveals that the tail inserts into the cavity, while a helix binds on the surface. Most contacts of Ro with the helix are to the backbone. Mutagenesis reveals that Ro has an extensive RNA-binding surface. We propose that Ro uses this surface to scavenge RNAs that fail to bind their specific RNA-binding proteins.
 ==About this Structure==
-I91 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with ACT and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2I91 OCA].
+I91 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I91 OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Xenopus laevis]]
-[[Category: Reinisch, K.M.]]
+[[Category: Reinisch, K M.]]
-[[Category: Stein, A.J.]]
+[[Category: Stein, A J.]]
 [[Category: ACT]]
 [[Category: MG]]
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 [[Category: von willebrand factor a]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:11:47 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:50:13 2008''