2ht6: Difference between revisions
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==Overview== | ==Overview== | ||
Gem, a member of the Rad,Gem/Kir subfamily of small G-proteins, has unique | Gem, a member of the Rad,Gem/Kir subfamily of small G-proteins, has unique sequence features. We report here the crystallographic structure determination of the Gem G-domain in complex with nucleotide to 2.4 A resolution. Although the basic Ras protein fold is maintained, the Gem switch regions emphatically differ from the Ras paradigm. Our ensuing biochemical characterization indicates that Gem G-domain markedly prefers GDP over GTP. Two known functions of Gem are distinctly affected by spatially separated clusters of mutations. | ||
==Disease== | ==Disease== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Hirsch, J | [[Category: Hirsch, J A.]] | ||
[[Category: Opatowsky, Y.]] | [[Category: Opatowsky, Y.]] | ||
[[Category: GDP]] | [[Category: GDP]] | ||
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[[Category: small g-protein]] | [[Category: small g-protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:45:26 2008'' | ||
Revision as of 18:45, 21 February 2008
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Crystal structure of Human Gem G-domain bound to GDP
OverviewOverview
Gem, a member of the Rad,Gem/Kir subfamily of small G-proteins, has unique sequence features. We report here the crystallographic structure determination of the Gem G-domain in complex with nucleotide to 2.4 A resolution. Although the basic Ras protein fold is maintained, the Gem switch regions emphatically differ from the Ras paradigm. Our ensuing biochemical characterization indicates that Gem G-domain markedly prefers GDP over GTP. Two known functions of Gem are distinctly affected by spatially separated clusters of mutations.
DiseaseDisease
Known diseases associated with this structure: Long QT syndrome-7 OMIM:[600681], Short QT syndrome-3 OMIM:[600681]
About this StructureAbout this Structure
2HT6 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structure-function studies of the G-domain from human gem, a novel small G-protein., Opatowsky Y, Sasson Y, Shaked I, Ward Y, Chomsky-Hecht O, Litvak Y, Selinger Z, Kelly K, Hirsch JA, FEBS Lett. 2006 Oct 30;580(25):5959-64. Epub 2006 Oct 6. PMID:17052716
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