2hoo: Difference between revisions

Revision as of 18:44, 21 February 2008

Crystal structure of an E. coli thi-box riboswitch bound to benfotiamine

OverviewOverview

Riboswitches are noncoding mRNA elements that bind small-molecule metabolites with high affinity and specificity, and they regulate the expression of associated genes. The thi-box riboswitch can exhibit a 1000-fold higher affinity for thiamine pyrophosphate over closely related noncognate compounds such as thiamine monophosphate. To understand the chemical basis of thi-box pyrophosphate specificity, we have determined crystal structures of an E. coli thi-box bound to thiamine pyrophosphate, thiamine monophosphate, and the structural analogs benfotiamine and pyrithiamine. When bound to monophosphorylated compounds, the RNA elements that recognize the thiamine and phosphate moieties of the ligand move closer together. This allows the riboswitch to recognize the monophosphate in a manner similar to how it recognizes the beta-phosphate of thiamine pyrophosphate. In the pyrithiamine complex, the pyrophosphate binding site is largely unstructured. These results show how the riboswitch can bind to various metabolites, and why the thi-box preferentially binds thiamine pyrophosphate.

About this StructureAbout this Structure

2HOO is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition., Edwards TE, Ferre-D'Amare AR, Structure. 2006 Sep;14(9):1459-68. PMID:16962976

Page seeded by OCA on Thu Feb 21 17:43:59 2008

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OCA

@@ Line 4: / Line 4: @@
 ==Overview==
-Riboswitches are noncoding mRNA elements that bind small-molecule, metabolites with high affinity and specificity, and they regulate the, expression of associated genes. The thi-box riboswitch can exhibit a, 1000-fold higher affinity for thiamine pyrophosphate over closely related, noncognate compounds such as thiamine monophosphate. To understand the, chemical basis of thi-box pyrophosphate specificity, we have determined, crystal structures of an E. coli thi-box bound to thiamine pyrophosphate, thiamine monophosphate, and the structural analogs benfotiamine and, pyrithiamine. When bound to monophosphorylated compounds, the RNA elements, that recognize the thiamine and phosphate moieties of the ligand move, closer together. This allows the riboswitch to recognize the monophosphate, in a manner similar to how it recognizes the beta-phosphate of thiamine, pyrophosphate. In the pyrithiamine complex, the pyrophosphate binding site, is largely unstructured. These results show how the riboswitch can bind to, various metabolites, and why the thi-box preferentially binds thiamine, pyrophosphate.
+Riboswitches are noncoding mRNA elements that bind small-molecule metabolites with high affinity and specificity, and they regulate the expression of associated genes. The thi-box riboswitch can exhibit a 1000-fold higher affinity for thiamine pyrophosphate over closely related noncognate compounds such as thiamine monophosphate. To understand the chemical basis of thi-box pyrophosphate specificity, we have determined crystal structures of an E. coli thi-box bound to thiamine pyrophosphate, thiamine monophosphate, and the structural analogs benfotiamine and pyrithiamine. When bound to monophosphorylated compounds, the RNA elements that recognize the thiamine and phosphate moieties of the ligand move closer together. This allows the riboswitch to recognize the monophosphate in a manner similar to how it recognizes the beta-phosphate of thiamine pyrophosphate. In the pyrithiamine complex, the pyrophosphate binding site is largely unstructured. These results show how the riboswitch can bind to various metabolites, and why the thi-box preferentially binds thiamine pyrophosphate.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition., Edwards TE, Ferre-D'Amare AR, Structure. 2006 Sep;14(9):1459-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16962976 16962976]
 [[Category: Protein complex]]
-[[Category: Amare, A.R.Ferre-D.]]
+[[Category: Amare, A R.Ferre-D.]]
-[[Category: Edwards, T.E.]]
+[[Category: Edwards, T E.]]
 [[Category: BFT]]
 [[Category: CA]]
 [[Category: rna; riboswitch; benfotiamine]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:25:39 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:43:59 2008''