2h4l: Difference between revisions
New page: left|200px<br /><applet load="2h4l" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h4l, resolution 2.40Å" /> '''Complex of PMM/PGM w... |
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[[Image:2h4l.gif|left|200px]]<br /><applet load="2h4l" size=" | [[Image:2h4l.gif|left|200px]]<br /><applet load="2h4l" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2h4l, resolution 2.40Å" /> | caption="2h4l, resolution 2.40Å" /> | ||
'''Complex of PMM/PGM with ribose 1-phosphate'''<br /> | '''Complex of PMM/PGM with ribose 1-phosphate'''<br /> | ||
==Overview== | ==Overview== | ||
Two complexes of the enzyme phosphomannomutase/phosphoglucomutase | Two complexes of the enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) from Pseudomonas aeruginosa with a slow substrate and with an inhibitor have been characterized by X-ray crystallography. Both ligands induce an interdomain rearrangement in the enzyme that creates a highly buried active site. Comparisons with enzyme-substrate complexes show that the inhibitor xylose 1-phosphate utilizes many of the previously observed enzyme-ligand interactions. In contrast, analysis of the ribose 1-phosphate complex reveals a combination of new and conserved enzyme-ligand interactions for binding. The ability of PMM/PGM to accommodate these two pentose phosphosugars in its active site may be relevant for future efforts towards inhibitor design. | ||
==About this Structure== | ==About this Structure== | ||
2H4L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with R1P and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2H4L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=R1P:'>R1P</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H4L OCA]. | ||
==Reference== | ==Reference== | ||
Complexes of the enzyme phosphomannomutase/phosphoglucomutase with a slow substrate and an inhibitor., Regni C, Shackelford GS, Beamer LJ, Acta | Complexes of the enzyme phosphomannomutase/phosphoglucomutase with a slow substrate and an inhibitor., Regni C, Shackelford GS, Beamer LJ, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):722-6. Epub 2006 Jul 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16880541 16880541] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Beamer, L | [[Category: Beamer, L J.]] | ||
[[Category: R1P]] | [[Category: R1P]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| Line 20: | Line 20: | ||
[[Category: slow substrate]] | [[Category: slow substrate]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:38:08 2008'' | ||
Revision as of 18:38, 21 February 2008
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Complex of PMM/PGM with ribose 1-phosphate
OverviewOverview
Two complexes of the enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) from Pseudomonas aeruginosa with a slow substrate and with an inhibitor have been characterized by X-ray crystallography. Both ligands induce an interdomain rearrangement in the enzyme that creates a highly buried active site. Comparisons with enzyme-substrate complexes show that the inhibitor xylose 1-phosphate utilizes many of the previously observed enzyme-ligand interactions. In contrast, analysis of the ribose 1-phosphate complex reveals a combination of new and conserved enzyme-ligand interactions for binding. The ability of PMM/PGM to accommodate these two pentose phosphosugars in its active site may be relevant for future efforts towards inhibitor design.
About this StructureAbout this Structure
2H4L is a Single protein structure of sequence from Pseudomonas aeruginosa with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Complexes of the enzyme phosphomannomutase/phosphoglucomutase with a slow substrate and an inhibitor., Regni C, Shackelford GS, Beamer LJ, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):722-6. Epub 2006 Jul 24. PMID:16880541
Page seeded by OCA on Thu Feb 21 17:38:08 2008