3vub: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
No edit summary
No edit summary
Line 1: Line 1:
{{Seed}}
[[Image:3vub.png|left|200px]]
<!--
The line below this paragraph, containing "STRUCTURE_3vub", creates the "Structure Box" on the page.
You may change the PDB parameter (which sets the PDB file loaded into the applet)
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
or leave the SCENE parameter empty for the default display.
-->
{{STRUCTURE_3vub|  PDB=3vub  |  SCENE=  }}  
{{STRUCTURE_3vub|  PDB=3vub  |  SCENE=  }}  
===CCDB, A TOPOISOMERASE POISON FROM E. COLI===
===CCDB, A TOPOISOMERASE POISON FROM E. COLI===
{{ABSTRACT_PUBMED_9917404}}


 
==Function==
<!--
[[http://www.uniprot.org/uniprot/CCDB_ECOLI CCDB_ECOLI]] Toxic component of a toxin-antitoxin (TA) module, functioning in plasmid maintainence. Responsible for the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Half-life of over 2 hours. Cell killing by CcdB is accompanied by filamentation, defects in chromosome and plasmid segregation, defects in cell division, formation of anucleate cells, decreased DNA synthesis and plasmid loss. Interferes with the activity of DNA gyrase, inducing it to form a covalent GyrA-DNA complex that cannot be resolved, thus promoting breakage of plasmid and chromosomal DNA. DNA breakage requires hydrolyzable ATP. Toxicity is inhibited by labile antitoxin CcdA, which blocks the activity of CcdB; CcdA also removes bound CcdB protein from the CcdB-GyrA complex by forming a CcdA-CcdB complex, a process termed rejuvenation. Also acts to inhibit partitioning of the chromosomal DNA. Functions as a transcriptional corepressor for the ccdAB operon, repression also requires CcdA.<ref>PMID:6327993</ref> <ref>PMID:2651399</ref> <ref>PMID:6308648</ref> <ref>PMID:2615761</ref> <ref>PMID:1324324</ref> <ref>PMID:8604132</ref> 
The line below this paragraph, {{ABSTRACT_PUBMED_9917404}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 9917404 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_9917404}}


==About this Structure==
==About this Structure==
3VUB is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUB OCA].  
[[3vub]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUB OCA].  


==Reference==
==Reference==
<ref group="xtra">PMID:9917404</ref><references group="xtra"/>
<ref group="xtra">PMID:009917404</ref><ref group="xtra">PMID:011604529</ref><ref group="xtra">PMID:011917145</ref><ref group="xtra">PMID:019006334</ref><references group="xtra"/><references/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Bahasi, E M.]]
[[Category: Bahasi, E M.]]
Line 34: Line 21:
[[Category: Wyns, L.]]
[[Category: Wyns, L.]]
[[Category: Ccdb]]
[[Category: Ccdb]]
[[Category: Plasmid]]
[[Category: Topoisomerase poison]]
[[Category: Topoisomerase poison]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:43:38 2009''

Revision as of 10:47, 16 May 2013

Template:STRUCTURE 3vub

CCDB, A TOPOISOMERASE POISON FROM E. COLICCDB, A TOPOISOMERASE POISON FROM E. COLI

Template:ABSTRACT PUBMED 9917404

FunctionFunction

[CCDB_ECOLI] Toxic component of a toxin-antitoxin (TA) module, functioning in plasmid maintainence. Responsible for the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Half-life of over 2 hours. Cell killing by CcdB is accompanied by filamentation, defects in chromosome and plasmid segregation, defects in cell division, formation of anucleate cells, decreased DNA synthesis and plasmid loss. Interferes with the activity of DNA gyrase, inducing it to form a covalent GyrA-DNA complex that cannot be resolved, thus promoting breakage of plasmid and chromosomal DNA. DNA breakage requires hydrolyzable ATP. Toxicity is inhibited by labile antitoxin CcdA, which blocks the activity of CcdB; CcdA also removes bound CcdB protein from the CcdB-GyrA complex by forming a CcdA-CcdB complex, a process termed rejuvenation. Also acts to inhibit partitioning of the chromosomal DNA. Functions as a transcriptional corepressor for the ccdAB operon, repression also requires CcdA.[1] [2] [3] [4] [5] [6]

About this StructureAbout this Structure

3vub is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

[xtra 1][xtra 2][xtra 3][xtra 4]

  1. Loris R, Dao-Thi MH, Bahassi EM, Van Melderen L, Poortmans F, Liddington R, Couturier M, Wyns L. Crystal structure of CcdB, a topoisomerase poison from E. coli. J Mol Biol. 1999 Jan 29;285(4):1667-77. PMID:9917404 doi:http://dx.doi.org/10.1006/jmbi.1998.2395
  2. Hayward S. Peptide-plane flipping in proteins. Protein Sci. 2001 Nov;10(11):2219-27. PMID:11604529 doi:10.1110/ps.23101
  3. Bhattacharyya R, Samanta U, Chakrabarti P. Aromatic-aromatic interactions in and around alpha-helices. Protein Eng. 2002 Feb;15(2):91-100. PMID:11917145
  4. Bajaj K, Dewan PC, Chakrabarti P, Goswami D, Barua B, Baliga C, Varadarajan R. Structural correlates of the temperature sensitive phenotype derived from saturation mutagenesis studies of CcdB. Biochemistry. 2008 Dec 9;47(49):12964-73. PMID:19006334 doi:10.1021/bi8014345
  1. Miki T, Yoshioka K, Horiuchi T. Control of cell division by sex factor F in Escherichia coli. I. The 42.84-43.6 F segment couples cell division of the host bacteria with replication of plasmid DNA. J Mol Biol. 1984 Apr 25;174(4):605-25. PMID:6327993
  2. Tam JE, Kline BC. Control of the ccd operon in plasmid F. J Bacteriol. 1989 May;171(5):2353-60. PMID:2651399
  3. Ogura T, Hiraga S. Mini-F plasmid genes that couple host cell division to plasmid proliferation. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4784-8. PMID:6308648
  4. Tam JE, Kline BC. The F plasmid ccd autorepressor is a complex of CcdA and CcdB proteins. Mol Gen Genet. 1989 Oct;219(1-2):26-32. PMID:2615761
  5. Bernard P, Couturier M. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. J Mol Biol. 1992 Aug 5;226(3):735-45. PMID:1324324
  6. Maki S, Takiguchi S, Horiuchi T, Sekimizu K, Miki T. Partner switching mechanisms in inactivation and rejuvenation of Escherichia coli DNA gyrase by F plasmid proteins LetD (CcdB) and LetA (CcdA). J Mol Biol. 1996 Mar 1;256(3):473-82. PMID:8604132 doi:http://dx.doi.org/10.1006/jmbi.1996.0102

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA