SB2013 L04gr5: Difference between revisions
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<scene name='SB2013_L04gr5/Ir6/1'>IR6</scene>, the second least exposed region composed of 26 amino acids, has been found to be the most conserved IR and the most immunogenic as observed in studies involving monkeys and humans. In a study conducted by Liang et al., serum samples from monkeys were infected by the bite of Ixodes scapularis nymphal ticks. The results showed a robust response to IR6 and little to no response to the remaining IRs. Similarly, only IR6 elicited a strong immune response in infected humans. Clearly, due to its highly conserved structure and immunodominance, IR6 is important to the functionality of B. Burgdorferi and therefore requires its strategic placement indicated in the crystallized structure (Eicken et al 2002). In mice, however, a strong response was detected across IRs 6, 2, and 4, but not in IRs 1, 3, and 5. (Liang et al 1999 A), thus demonstrating the importance of IR6 across many species and indicating that further research into the fuctions of IRs 2 and 4 is needed. | <scene name='SB2013_L04gr5/Ir6/1'>IR6</scene>, the second least exposed region composed of 26 amino acids, has been found to be the most conserved IR and the most immunogenic as observed in studies involving monkeys and humans. In a study conducted by Liang et al., serum samples from monkeys were infected by the bite of Ixodes scapularis nymphal ticks. The results showed a robust response to IR6 and little to no response to the remaining IRs. Similarly, only IR6 elicited a strong immune response in infected humans. Clearly, due to its highly conserved structure and immunodominance, IR6 is important to the functionality of B. Burgdorferi and therefore requires its strategic placement indicated in the crystallized structure (Eicken et al 2002). In mice, however, a strong response was detected across IRs 6, 2, and 4, but not in IRs 1, 3, and 5. (Liang et al 1999 A), thus demonstrating the importance of IR6 across many species and indicating that further research into the fuctions of IRs 2 and 4 is needed. | ||
The high immunogenicity effect of IR6 is thought to only occur in dead bacteria due to its low maximal theoretical surface exposure in living B. borgderfuri (13.7%). Interaction with anti-IR6 antibodies would be limited to the exposed <scene name='SB2013_L04gr5/Ir6_residues/1'>amino acid residues</scene>, Lys-274, Gln-279, Lys-291,Lys-294 (Eicken). These amino acids would be the likely targets of the host immune response. The immunodominant nature of VlsE, especially in IR6, makes it viable diagnostic tool (Marangoni). | The high immunogenicity effect of IR6 is thought to only occur in dead bacteria due to its low maximal theoretical surface exposure in living B. borgderfuri (13.7%). Interaction with anti-IR6 antibodies would be limited to the exposed <scene name='SB2013_L04gr5/Ir6_residues/1'>amino acid residues</scene>, Lys-274, Gln-279, Lys-291,Lys-294 (Eicken). These amino acids would be the likely targets of the host immune response. The immunodominant nature of VlsE, especially in IR6, makes it a viable diagnostic tool (Marangoni). | ||
<scene name='SB2013_L04gr5/Reset_original/2'>Reset Structure</scene> | <scene name='SB2013_L04gr5/Reset_original/2'>Reset Structure</scene> | ||