Group:MUZIC:actinin2: Difference between revisions

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===α-actinin-2===

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The solution structure of the complex between the human α-actinin-2 EF-hand domain and the chicken titin Z-repeat 7 domain was solved by NMR spectroscopy. A specific set of contacts and some important residues for protein-protein interactions were identified. It was shown that recognition and positioning of Z-repeat in cavity of EF-hand domain is mediated by balance of electrostatic and hydrophobic interactions. The reported semi-open conformation of complex is commonly seen in homologous structures. This conformation seems to be typical for calcium-independent recognition by EF-hand domain and is fully compatible with formation of stable long-term complexes between titin and α-actinin-2.

~~<Structure load='1TJT' size='200' frame='true' align='right' caption='Fig. 1- Crystal structure of α-actinin-3 ABD domain [[1tjt]]' scene='Insert optional scene name here' />~~ <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Actin_binding_domain/11'>~~(Fig. 1)~~</scene>

* <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Actin_binding_domain/11'>Crystal structure of alpha-actinin-3 ABD domain</scene> ([[1tjt]])

~~<Structure load='1HCI' size='200' frame='true' align='right' caption='Fig. 2 - Crystal structure of rod domain of α-actinin-2 [[1hci]]' scene='insert optional scene name here' />~~ <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Rod_domain_sr_dimer/3'>~~(Fig.~~ 2)</scene>

* <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Rod_domain_sr_dimer/3'>Crystal structure of rod domain of alpha-actinin-2</scene> ([[1hci]])

<~~Structure load~~='~~1H8B~~ ' ~~size='200' frame='true' align='right' caption='Fig. 3 -~~ NMR structure of α-actinin-2 EF-hand domain:chicken titin Z-repeat 7 domain complex [[1h8b]]~~' scene='insert optional scene name here' /> <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Efhand34_zr7titin/2'>(Fig. 3~~)~~</scene>~~

* <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Efhand34_zr7titin/2'>NMR structure of α-actinin-2 EF-hand domain:chicken titin Z-repeat 7 domain complex</scene> ([[1h8b]])

==Pathology==

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+<StructureSection load='' size='1tjt' side='right' scene='' caption=''>
 ===α-actinin-2===
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 The solution structure of the complex between the human α-actinin-2 EF-hand domain and the chicken titin Z-repeat 7 domain was solved by NMR spectroscopy. A specific set of contacts and some important residues for protein-protein interactions were identified. It was shown that recognition and positioning of Z-repeat in cavity of EF-hand domain is mediated by balance of electrostatic and hydrophobic interactions. The reported semi-open conformation of complex is commonly seen in homologous structures. This conformation seems to be typical for calcium-independent recognition by EF-hand domain and is fully compatible with formation of stable long-term complexes between titin and α-actinin-2.
-<Structure load='1TJT' size='200' frame='true' align='right' caption='Fig. 1- Crystal structure of α-actinin-3 ABD domain [[1tjt]]' scene='Insert optional scene name here' /> <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Actin_binding_domain/11'>(Fig. 1)</scene>
+* <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Actin_binding_domain/11'>Crystal structure of alpha-actinin-3 ABD domain</scene> ([[1tjt]])
-<Structure load='1HCI' size='200' frame='true' align='right' caption='Fig. 2 - Crystal structure of rod domain of α-actinin-2 [[1hci]]' scene='insert optional scene name here' /> <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Rod_domain_sr_dimer/3'>(Fig. 2)</scene>
+* <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Rod_domain_sr_dimer/3'>Crystal structure of rod domain of alpha-actinin-2</scene> ([[1hci]])
-<Structure load='1H8B ' size='200' frame='true' align='right' caption='Fig. 3 - NMR structure of α-actinin-2 EF-hand domain:chicken titin Z-repeat 7 domain complex [[1h8b]]' scene='insert optional scene name here' /> <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Efhand34_zr7titin/2'>(Fig. 3)</scene>
+* <scene name='User:Euripides_Ribeiro/workbench/alpha-actinin/Efhand34_zr7titin/2'>NMR structure of α-actinin-2 EF-hand domain:chicken titin Z-repeat 7 domain complex</scene> ([[1h8b]])
 ==Pathology==